Selective Quantification of Chemotropic Responses of Fusarium graminearum

Pooja S Sridhar; Tanya Sharma; Michele C Loewen

doi:10.1007/978-1-0716-3159-1_5

Selective Quantification of Chemotropic Responses of Fusarium graminearum

Methods Mol Biol. 2023:2659:61-71. doi: 10.1007/978-1-0716-3159-1_5.

Authors

Pooja S Sridhar¹, Tanya Sharma², Michele C Loewen³

Affiliations

¹ Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.
² Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON, Canada.
³ Aquatic and Crop Resources Development Research Center, National Research Council of Canada, Ottawa, ON, Canada. Michele.Loewen@nrc-cnrc.gc.ca.

PMID: 37249885
DOI: 10.1007/978-1-0716-3159-1_5

Abstract

Chemotropism refers to the directional growth of a living organism toward a chemical stimulus. Molecular mechanisms underlying chemotropism of fungal pathogens have recently been enabled by advancements in biological chemotropic assays, with a particular focus on the roles of G-protein-coupled receptors and their plant-derived ligands in chemotropism. Here we describe in detail an assay that enables quantification of chemotropic responses of Fusarium graminearum, with variations recently reported for Fusarium oxysporum and Trichoderma atroviride.

Keywords: Chemotropic index; Chemotropism; Directed hyphal growth; Fusarium graminearum; Hyphal length; Microscopy; Plate assay.

MeSH terms

Chemotaxis*
Fusarium*
Plant Diseases / microbiology
Plants
Receptors, G-Protein-Coupled

Substances

Receptors, G-Protein-Coupled

Supplementary concepts

Fusarium graminearum