Viral Transduction of Mammalian Spermatogonial Stem Cells

Makiko Yamada; Yanyun Luo; Marco Seandel

doi:10.1007/978-1-0716-3139-3_12

Viral Transduction of Mammalian Spermatogonial Stem Cells

Methods Mol Biol. 2023:2656:211-225. doi: 10.1007/978-1-0716-3139-3_12.

Authors

Makiko Yamada¹, Yanyun Luo¹, Marco Seandel²

Affiliations

¹ Department of Surgery, Weill Cornell Medicine, New York, NY, USA.
² Department of Surgery, Weill Cornell Medicine, New York, NY, USA. mas9066@med.cornell.edu.

PMID: 37249874
DOI: 10.1007/978-1-0716-3139-3_12

Abstract

Lentiviral vectors have been major tools for genetic manipulation of spermatogonial stem cells (SSCs) in vitro. Adeno-associated viral vectors are promising emerging tools for in vivo SSC transduction that are less invasive, compared to lentivirus, since AAV DNA is not integrated into the host genome and the host genome remains intact. In this chapter, we describe protocols using lentiviral and adeno-associated viral vectors to transduce SSCs in vitro and vivo, respectively.

Keywords: Adeno-associated virus; Lentivirus; Mouse; Seminiferous tubule; Testis; Transgenesis.

MeSH terms

Animals
Genetic Techniques*
Genetic Vectors / genetics
Lentivirus / genetics
Male
Mammals* / genetics
Spermatogonia
Stem Cells
Transduction, Genetic