In this work, our new experimental setup has been used to study the ionization and fragmentation of a prebiotic molecule, hydantoin, by electron impact. Scanning of the incident electron energy allows the determination of the appearance thresholds of the cations. The vertical ionization potential was found to be in good agreement with previous data. Dissociation thresholds for the main fragmentation patterns were also measured. In parallel, thanks to quantum chemical calculations, reaction schemes compatible with the experimental results are given.