Dual roles of CYP302A1 in regulating ovarian maturation and molting in Macrobrachium nipponense

Yalu Zheng; Wenyi Zhang; Yiwei Xiong; Jisheng Wang; Shubo Jin; Hui Qiao; Sufei Jiang; Hongtuo Fu

doi:10.1016/j.jsbmb.2023.106336

Dual roles of CYP302A1 in regulating ovarian maturation and molting in Macrobrachium nipponense

J Steroid Biochem Mol Biol. 2023 Sep:232:106336. doi: 10.1016/j.jsbmb.2023.106336. Epub 2023 May 27.

Authors

Yalu Zheng¹, Wenyi Zhang², Yiwei Xiong², Jisheng Wang¹, Shubo Jin², Hui Qiao³, Sufei Jiang⁴, Hongtuo Fu⁵

Affiliations

¹ Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
² Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
³ Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. Electronic address: qiaoh@ffrc.cn.
⁴ Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. Electronic address: jiangsf@ffrc.cn.
⁵ Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. Electronic address: fuht@ffrc.cn.

PMID: 37247747
DOI: 10.1016/j.jsbmb.2023.106336

Abstract

CYP302A1 is a member of the Halloween genes in the cytochrome P450 supergene family, which play an important regulatory role in the synthesis of 20-hydroxyecdysone (20E) in crustaceans and insects. In this study, we found that the Mn-CYP302A1sequence included typical CYP450 conserved domains. Phylogenic showed that it is closely related to crustaceans and insects. q-PCR analysis indicated that Mn-CYP302A1 was highly expressed in the ovaries and peaked before ovarian maturation. Mn-CYP302A1 expression was higher at the post-larval stage of day 15 than at other stages of embryogenesis. In situ hybridization indicated that Mn-CYP302A1 was mainly distributed in the nucleus, yolk granules, cell membrane and cytoplasm To further establish the function of CYP302A1, a 21-day RNA interference experiment was conducted. On day 16, the Gonad Somatic Index of the control group and the experimental group showed significant differences, with GSI of 11.72% in the control group and 3.21% in the experimental group. The cumulative proportion of the second entry into stage O-Ⅲ was 100% in the control group, while it was 41.67% in the experimental group on day 21. The ecdysone content was 8.91nmol/L in the control group and 6.11nmol/L in the experimental group on day 9. A significant difference in the molting proportion between the control group and the experimental group was also observed (49% in the control group and 34% in the experimental group) on day 16. Statistical results showed that the average molting cycle of the control group was 14.5 days, while that of the experimental group was 16.5 days. However, the morphological structure of ovarian tissue did not abnormal change. Therefore, the results of this study suggest that Mn-CYP302A1 can promote ovarian maturation and molting in female M. nipponense.

Keywords: CYP302A1; Macrobrachium nipponense; Molting; Ovarian maturation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Ecdysone / metabolism
Female
Insecta
Molting* / genetics
Palaemonidae* / genetics
Sex Differentiation

Substances

Ecdysone