Ps-Pt nanozyme-based synergistic signal amplification biosensor for highly sensitive colorimetric detection of protein

Chaohui Chen; Mengting Qi; Cheng Fu; Rongxiang He; Long Chen; Jiao Hu

doi:10.1016/j.talanta.2023.124700

Ps^-Pt nanozyme-based synergistic signal amplification biosensor for highly sensitive colorimetric detection of protein

Talanta. 2023 Oct 1:263:124700. doi: 10.1016/j.talanta.2023.124700. Epub 2023 May 26.

Authors

Chaohui Chen¹, Mengting Qi², Cheng Fu³, Rongxiang He³, Long Chen⁴, Jiao Hu⁵

Affiliations

¹ State Key Laboratory of Precision Blasting, Jianghan University, Wuhan 430056, PR China; Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, College of Photoelectric Materials and Technology, Jianghan University, Wuhan 430056, Hubei, China. Electronic address: chaohui20071220@163.com.
² Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, College of Photoelectric Materials and Technology, Jianghan University, Wuhan 430056, Hubei, China.
³ State Key Laboratory of Precision Blasting, Jianghan University, Wuhan 430056, PR China; Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, College of Photoelectric Materials and Technology, Jianghan University, Wuhan 430056, Hubei, China.
⁴ Department of Respiratory and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.
⁵ State Key Laboratory of Precision Blasting, Jianghan University, Wuhan 430056, PR China; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances, School of Environment and Health, Jianghan University, Wuhan, 430056, Hubei, China. Electronic address: hujiao@whu.edu.cn.

PMID: 37247452
DOI: 10.1016/j.talanta.2023.124700

Abstract

Immunosorbent assay is one of the most popular immunological screening techniques which has been widely used for the clinical diagnosis of alpha-fetoprotein (AFP). While traditional immunosorbent assay (ELISA) suffers from low detection sensitivity due to its low intensity of colorimetric signal. To improve the sensitivity of AFP detection, we developed a new and sensitive immunocolorimetric biosensor by combining Ps-Pt nanozyme with terminal deoxynucleotidyl transferase (TdT)-mediated polymerization reaction. The determination of AFP was achieved by measuring the visual color intensity produced by the catalytic oxidation reaction of the 3,3',5,5'-tetramethylbenzidine (TMB) solution with Ps-Pt and horseradish peroxidase (HRP). Owing to the synergistic catalysis of Ps-Pt and horseradish peroxidase HRP enriched in polymerized amplification products, this biosensor exhibited a significant color change within 25 s in the presence of 10-500 pg/mL AFP. This proposed method allowed for the specific detection of AFP with a detection limit of 4.30 pg/mL and even 10 pg/mL target protein could be distinguished clearly by visual observation. Furthermore, this biosensor could be applied to analysis of AFP in the complex sample and could be easily extended to the detection of other proteins.

Keywords: Colorimetric; Protein detection; Ps-Pt; Synergistic amplification.

MeSH terms

Biosensing Techniques* / methods
Colorimetry / methods
Horseradish Peroxidase / metabolism
Hydrogen Peroxide
Immunosorbents
Limit of Detection
alpha-Fetoproteins* / analysis

Substances

alpha-Fetoproteins
Immunosorbents
Horseradish Peroxidase
Hydrogen Peroxide