High mobility group box 1 in women with unexplained recurrent pregnancy loss

Carlo Ticconi; Stefania Mardente; Emanuela Mari; Federica Barreca; Manuela Montanaro; Alessandro Mauriello; Giuseppe Rizzo; Alessandra Zicari

doi:10.1515/jpm-2023-0109

High mobility group box 1 in women with unexplained recurrent pregnancy loss

J Perinat Med. 2023 May 30;51(9):1139-1146. doi: 10.1515/jpm-2023-0109. Print 2023 Nov 27.

Authors

Carlo Ticconi¹, Stefania Mardente¹, Emanuela Mari², Federica Barreca², Manuela Montanaro³, Alessandro Mauriello³, Giuseppe Rizzo⁴, Alessandra Zicari²

Affiliations

¹ Department of Surgical Sciences, Section of Gynecology and Obstetrics, University of Rome "Tor Vergata", Rome, Italy.
² Department of Experimental Medicine, University of Rome "La Sapienza", Rome, Italy.
³ Department of Experimental Medicine, University of Rome "Tor Vergata", Rome, Italy.
⁴ Department of Biomedicine and Prevention, Section of Gynecology and Obstetrics, University of Rome "Tor Vergata", Rome, Italy.

PMID: 37246521
DOI: 10.1515/jpm-2023-0109

Abstract

Objectives: To investigate whether high mobility group box 1 (HMGB1) is involved in unexplained recurrent pregnancy loss (uRPL).

Methods: Plasma levels of HMGB1 were measured by ELISA in non-pregnant women with (n=44) and without (n=53 controls) uRPL. Their platelets and plasma-derived microvesicles (MVs) were also assayed for HMGB1. Endometrial biopsies were taken in selected uRPL (n=5) and control women (n=5) and the tissue expression of HMGB1 was determined by western blot and immunohistochemistry (IHC).

Results: plasma levels of HMGB1 were significantly higher in women with uRPL than in control women. HMGB1 content in platelets and MVs obtained from women with uRPL was significantly higher than that obtained from control women. HMGB1 expression in endometrium was higher in tissues obtained from women with uRPL than in tissues obtained from control women. IHC analysis revealed that HMGB1 is expressed in endometrium with different patterns between uRPL and control women.

Conclusions: HMGB1 could be involved in uRPL.

Keywords: endometrium; high mobility group box 1 (HMGB1); microvesicles; recurrent pregnancy loss.

MeSH terms

Abortion, Habitual*
Endometrium
Enzyme-Linked Immunosorbent Assay
Female
HMGB1 Protein* / metabolism
Humans
Pregnancy

Substances

HMGB1 Protein