Spectral deconvolution of electron-bifurcating flavoproteins

Steve Ortiz; Dimitri Niks; Wayne Vigil Jr; Jessica Tran; Carolyn E Lubner; Russ Hille

doi:10.1016/bs.mie.2023.03.011

Spectral deconvolution of electron-bifurcating flavoproteins

Methods Enzymol. 2023:685:531-550. doi: 10.1016/bs.mie.2023.03.011. Epub 2023 Apr 19.

Authors

Steve Ortiz¹, Dimitri Niks¹, Wayne Vigil Jr¹, Jessica Tran¹, Carolyn E Lubner², Russ Hille³

Affiliations

¹ Department of Biochemistry, University of California, Riverside, United States.
² Biosciences Center, National Renewable Energy Laboratory, Golden, CO, United States. Electronic address: cara.lubner@nrel.gov.
³ Department of Biochemistry, University of California, Riverside, United States. Electronic address: russ.hille@ucr.edu.

PMID: 37245914
DOI: 10.1016/bs.mie.2023.03.011

Abstract

Electron-bifurcating flavoproteins catalyze the tightly coupled reduction of high- and low-potential acceptors using a median-potential electron donor, and are invariably complex systems with multiple redox-active centers in two or more subunits. Methods are described that permit, in favorable cases, the deconvolution of spectral changes associated with reduction of specific centers, making it possible to dissect the overall process of electron bifurcation into individual, discrete steps.

Keywords: Electron bifurcation; Electron paramagnetic resonance; Flavoproteins; Transient absorbance spectroscopy.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural

MeSH terms

Catalysis
Electron Transport
Electrons*
Flavoproteins*
Oxidation-Reduction

Substances

Flavoproteins