Rapid detection of foodborne pathogens in diverse foodstuffs by universal electrochemical aptasensor based on UiO-66 and methylene blue composites

Ge Dai; Handong Yao; Liuhong Yang; Yifeng Ding; Shuxin Du; Hui Shen; Fengfeng Mo

doi:10.1016/j.foodchem.2023.136244

Rapid detection of foodborne pathogens in diverse foodstuffs by universal electrochemical aptasensor based on UiO-66 and methylene blue composites

Food Chem. 2023 Oct 30:424:136244. doi: 10.1016/j.foodchem.2023.136244. Epub 2023 May 11.

Authors

Ge Dai¹, Handong Yao², Liuhong Yang³, Yifeng Ding³, Shuxin Du⁴, Hui Shen⁵, Fengfeng Mo⁶

Affiliations

¹ Department of Naval Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, 800 Xiangyin Road, Shanghai 200433, China.
² Department of Naval Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, 800 Xiangyin Road, Shanghai 200433, China; School of Engineering, Huzhou University, Huzhou 313000, China.
³ Department of Naval Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, 800 Xiangyin Road, Shanghai 200433, China; School of Health Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, China.
⁴ School of Engineering, Huzhou University, Huzhou 313000, China.
⁵ Department of Naval Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, 800 Xiangyin Road, Shanghai 200433, China. Electronic address: shenhuicn@126.com.
⁶ Department of Naval Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, 800 Xiangyin Road, Shanghai 200433, China. Electronic address: mofengfeng@smmu.edu.cn.

PMID: 37244183
DOI: 10.1016/j.foodchem.2023.136244

Abstract

Rapid and sensitive detection of foodborne pathogens in complex environments is essential for food protection. A universal electrochemical aptasensor was fabricated for the detection of three common foodborne pathogens, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Salmonella typhimurium (S. typhimurium). The aptasensor was developed based on the homogeneous and membrane filtration strategy. Zirconium-based metal-organic framework (UiO-66)/methylene blue (MB)/aptamer composite was designed as a signal amplification and recognition probe. Bacteria were quantitatively detected by the current changes of MB. By simply changing the aptamer, different bacteria could be detected. The detection limits of E. coli, S. aureus and S. typhimurium were 5, 4 and 3 CFU·mL^-1, respectively. In humidity and salt environments, the stability of the aptasensor was satisfactory. The aptasensor exhibited satisfactory detection performance in different real samples. This aptasensor has excellent potential for rapid detection of foodborne pathogens in complex environments.

Keywords: Diverse foodstuffs; Foodborne pathogens; Homogeneous electrochemical aptasensor; Membrane filtration; UiO-66/MB/aptamer.

MeSH terms

Aptamers, Nucleotide* / chemistry
Biosensing Techniques*
Electrochemical Techniques
Escherichia coli
Gold / chemistry
Limit of Detection
Methylene Blue / chemistry
Staphylococcus aureus

Substances

UiO-66
Methylene Blue
Aptamers, Nucleotide
Gold