Retrospective phylogenetic analysis of rabies virus G and N genes from Serbia

Abstract

Rabies is a viral disease of the central nervous system of all warm-blooded animals and one of the oldest and most important zoonoses. In the Republic of Serbia, rabies is controlled by compulsory vaccination of dogs and cats and oral vaccination of wild carnivores, which has been implemented since 2010. In the period 2009-2018, 367 rabies cases were recorded. The last rabies case in Serbia was recorded in 2018 in a red fox (Vulpes vulpes), while the last case in dogs was diagnosed in 2011. This study aimed to perform a retrospective phylogenetic analysis of G and N genes of the rabies virus from Serbia from 2009 to 2018 to understand sources and pathways of infection better and to enable molecular virus tracing in the future based on the association of rabies virus genetic lineages with the geographic distribution. For the phylogenetic analysis of the rabies virus, 14 historically positive brain samples of red foxes from 2009 to 2018, collected in central Serbia, were used. All field strains from Serbia were identified as classic rabies virus and grouped within the Cosmopolitan lineage. Phylogenetic analysis of N gene sequences revealed that 13 rabies virus strains (92.9%) from Serbia belonged to the EE sub-lineage of isolates, while one virus (7.1%) ON988027 from 2011 clustered together with isolates from the WE sub-lineage.

Keywords: G gene; N gene; Serbia; phylogenetic analysis; rabies virus.