Genome-Wide Identification and Functional Analysis of NAP1 in Triticum aestivum

Huimin Feng; Mila Wu; Ziqiong Wang; Xia Wang; Jianping Chen; Jian Yang; Peng Liu

doi:10.3390/genes14051041

Genome-Wide Identification and Functional Analysis of NAP1 in Triticum aestivum

Genes (Basel). 2023 May 4;14(5):1041. doi: 10.3390/genes14051041.

Authors

Huimin Feng^{1

2}, Mila Wu^{1

2}, Ziqiong Wang^{1

2}, Xia Wang^{1

2}, Jianping Chen^{1

2}, Jian Yang¹, Peng Liu^{1

2}

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
² Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.

Abstract

As a main molecular chaperone of histone H2A-H2B, nucleosome assembly protein 1 (NAP1) has been widely researched in many species. However, there is little research investigating the function of NAP1 in Triticum aestivum. To understand the capabilities of the family of NAP1 genes in wheat and the relationship between TaNAP1 genes and plant viruses, we performed comprehensive genome-wide analysis and quantitative real-time polymerase chain reaction (qRT-PCR) for testing expression profiling under hormonal and viral stresses. Our results showed that TaNAP1 was expressed at different levels in different tissues, with higher expression in tissues with high meristematic capacity, such as roots. Furthermore, the TaNAP1 family may participate in plant defense mechanisms. This study provides a systematic analysis of the NAP1 gene family in wheat and lays the foundation for further studies on the function of TaNAP1 in the response of wheat plants to viral infection.

Keywords: NAP1; plant gene expression; plant immunity; plant viruses; wheat.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Genome, Plant
Molecular Chaperones / genetics
Nucleosome Assembly Protein 1* / genetics
Nucleosome Assembly Protein 1* / metabolism
Triticum* / genetics
Triticum* / metabolism

Substances

Nucleosome Assembly Protein 1
nucleic acid probe 1
Molecular Chaperones

Grants and funding

This research received the Science & Technology Public Welfare Project of Ningbo City, China (202002N3004), Agriculture Research System from the Ministry of Agriculture of the P.R. China (CARS-03), the Natural Science Foundation of Zhejiang province (LDQ23C140001) and National Natural Science Foundation of China (32100126).