Recent advances in N- and C-terminus cysteine protein bioconjugation

Richard J Spears; Vijay Chudasama

doi:10.1016/j.cbpa.2023.102306

Recent advances in N- and C-terminus cysteine protein bioconjugation

Curr Opin Chem Biol. 2023 Aug:75:102306. doi: 10.1016/j.cbpa.2023.102306. Epub 2023 May 24.

Authors

Richard J Spears¹, Vijay Chudasama²

Affiliations

¹ Department of Chemistry, University College London, 20 Gordon Street, London, UK.
² Department of Chemistry, University College London, 20 Gordon Street, London, UK. Electronic address: v.chudasama@ucl.ac.uk.

PMID: 37236135
DOI: 10.1016/j.cbpa.2023.102306

Abstract

Advances in the site-specific chemical modification of proteins, also referred to as protein bioconjugation, have proved instrumental in revolutionary approaches to designing new protein-based therapeutics. Of the sites available for protein modification, cysteine residues or the termini of proteins have proved especially popular owing to their favorable properties for site-specific modification. Strategies that, therefore, specifically target cysteine at the termini offer a combination of these favorable properties of cysteine and termini bioconjugation. In this review, we discuss these strategies with a particular focus on those reported recently and provide our opinion on the future direction of the field.

Keywords: C-terminal modification; Cysteine conjugation; N-terminal modification; Peptide modification; Protein modification; Termini modification.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biochemistry* / methods
Cysteine* / chemistry
Proteins* / chemistry
Staining and Labeling / methods
Thiazoles / chemistry

Substances

Proteins
Cysteine
Thiazoles