Multi-Reconfigurable DNA Origami Nanolattice Driven by the Combination of Orthogonal Signals

Kotaro Watanabe; Ibuki Kawamata; Satoshi Murata; Yuki Suzuki

doi:10.1021/jacsau.3c00091

Multi-Reconfigurable DNA Origami Nanolattice Driven by the Combination of Orthogonal Signals

JACS Au. 2023 Apr 27;3(5):1435-1442. doi: 10.1021/jacsau.3c00091. eCollection 2023 May 22.

Authors

Kotaro Watanabe¹, Ibuki Kawamata¹, Satoshi Murata¹, Yuki Suzuki^{2

3}

Affiliations

¹ Department of Robotics, Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki-Aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
² Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, 6-3 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8578, Japan.
³ Department of Chemistry for Materials, Graduate School of Engineering, Mie University, 1577 Kurimamachiya-Cho, Tsu 514-8507, Mie, Japan.

Abstract

The progress of the scaffolded DNA origami technology has enabled the construction of various dynamic nanodevices imitating the shapes and motions of mechanical elements. To further expand the achievable configurational changes, the incorporation of multiple movable joints into a single DNA origami structure and their precise control are desired. Here, we propose a multi-reconfigurable 3 × 3 lattice structure consisting of nine frames with rigid four-helix struts connected with flexible 10-nucleotide joints. The configuration of each frame is determined by the arbitrarily selected orthogonal pair of signal DNAs, resulting in the transformation of the lattice into various shapes. We also demonstrated sequential reconfiguration of the nanolattice and its assemblies from one into another via an isothermal strand displacement reaction at physiological temperatures. Our modular and scalable design approach could serve as a versatile platform for a variety of applications that require reversible and continuous shape control with nanoscale precision.