Degradation of xylan by human gut Bacteroides xylanisolvens XB1A

Tingting Zhao; Han Yue; Junfeng Peng; Yingmin Nie; Longzhen Wu; Tingting Li; Wei Niu; Chuan Li; Zhengqing Zhang; Meixia Li; Kan Ding

doi:10.1016/j.carbpol.2023.121005

Degradation of xylan by human gut Bacteroides xylanisolvens XB1A

Carbohydr Polym. 2023 Sep 1:315:121005. doi: 10.1016/j.carbpol.2023.121005. Epub 2023 May 11.

Authors

Tingting Zhao¹, Han Yue², Junfeng Peng³, Yingmin Nie⁴, Longzhen Wu², Tingting Li², Wei Niu², Chuan Li², Zhengqing Zhang⁵, Meixia Li⁶, Kan Ding⁷

Affiliations

¹ School of Chinese Materia Medica, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, Nanjing, Jiangsu Province 210029, PR China; Carbohydrate-Based Drug Research Center, CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China.
² Carbohydrate-Based Drug Research Center, CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China.
³ Department of Pancreatic-biliary Surgery, Naval Medical University, Shanghai, PR China.
⁴ Carbohydrate-Based Drug Research Center, CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China; University of Chinese Academy of Science, No.19A Yuquan Road, Beijing 100049, PR China.
⁵ Jiangsu Key Laboratory of Neuropsychiatric Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, PR China. Electronic address: z_zhang@suda.edu.cn.
⁶ Carbohydrate-Based Drug Research Center, CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China. Electronic address: limei@simm.ac.cn.
⁷ School of Chinese Materia Medica, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, Nanjing, Jiangsu Province 210029, PR China; Carbohydrate-Based Drug Research Center, CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China; University of Chinese Academy of Science, No.19A Yuquan Road, Beijing 100049, PR China; Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Science, SSIP Healthcare and Medicine Demonstration Zone, Zhongshan Tsuihang New District, Zhongshan 528400, PR China. Electronic address: dingkan@simm.ac.cn.

PMID: 37230606
DOI: 10.1016/j.carbpol.2023.121005

Abstract

Although many polysaccharides utilization loci (PULs) have been investigated by genomics and transcriptomics, the detailed functional characterization lags severely behind. We hypothesize that PULs on the genome of Bacteroides xylanisolvens XB1A (BX) dictate the degradation of complex xylan. To address, xylan S32 isolated from Dendrobium officinale was employed as a sample polysaccharide. We firstly showed that xylan S32 promoted the growth of BX which might degrade xylan S32 into monosaccharides and oligosaccharides. We further showed that this degradation was performed mainly via two discrete PULs in the genome of BX. Briefly, a new surface glycan binding protein (SGBP) BX_29290^SGBP was identified, and shown to be essential for the growth of BX on xylan S32. Two cell surface endo-xylanases Xyn10A and Xyn10B cooperated to deconstruct the xylan S32. Intriguingly, genes encoding Xyn10A and Xyn10B were mainly distributed in the genome of Bacteroides spp. In addition, BX metabolized xylan S32 to produce short chain fatty acids (SCFAs) and folate. Taken together, these findings provide new evidence to understand the food source of BX and the BX-directed intervention strategy by xylan.

Keywords: Bacteroides xylanisolvens XB1A; Degradation; Dendrobium officinale; Xylan.

MeSH terms

Bacteroides / genetics
Bacteroides / metabolism
Gene Expression Profiling
Humans
Polysaccharides* / metabolism
Xylans* / metabolism

Substances

Xylans
Polysaccharides

Supplementary concepts

Bacteroides xylanisolvens