Bioprocess engineering to produce essential polyunsaturated fatty acids from Thraustochytrium sp

Ajeet Singh Chauhan; Chiu-Wen Chen; Vaibhav Sunil Tambat; Reeta Rani Singhania; Jo-Shu Chang; Cheng-Di Dong; Anil Kumar Patel

doi:10.1016/j.biortech.2023.129209

Bioprocess engineering to produce essential polyunsaturated fatty acids from Thraustochytrium sp

Bioresour Technol. 2023 Sep:383:129209. doi: 10.1016/j.biortech.2023.129209. Epub 2023 May 23.

Authors

Ajeet Singh Chauhan¹, Chiu-Wen Chen², Vaibhav Sunil Tambat¹, Reeta Rani Singhania³, Jo-Shu Chang⁴, Cheng-Di Dong⁵, Anil Kumar Patel³

Affiliations

¹ Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan.
² Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan.
³ Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Centre for Energy and Environmental Sustainability, Lucknow 226 029, Uttar Pradesh, India.
⁴ Department of Chemical and Materials Engineering, Tunghai University, Taiwan; Research Center for Smart Sustainable Circular Economy, Tunghai University, Taiwan; Department of Chemical Engineering, National Cheng Kung University, Taiwan.
⁵ Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan. Electronic address: cddong@nkust.edu.tw.

PMID: 37230331
DOI: 10.1016/j.biortech.2023.129209

Abstract

In recent studies, thraustochytrid has emerged as a sustainable substitute to fish oil or polyunsaturated fatty acid (PUFA) sources: docosapentaenoic acid (DPA) eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Due to growing health concerns, there is increasing demand for food and health applications of PUFA for several diseases, aquaculture feeds, and dietary products. Thraustochytrium sp. found a sustainable source for considerable PUFA and SFA production and to meet omega PUFA demand globally. This study aims to increase PUFA yield by the maximum possible glucose carbon with an appropriate nitrogen ratio (10:1). The maximum biomass and lipid obtained from 40 g/L glucose, were 7.47 ± 0.3 g/L and 4.63 g/L (60.84 ± 1.4%), respectively. However, maximum relative lipid, DHA and DPA yields were from 30 g/L glucose i.e, 67.6 ± 1.9 % and 963.58 ± 24 and 693.10 ± 24 mg/L respectively with complete glucose assimilation. Thus, this could be a potential source of commercial DPA and DHA producers under the biorefinery scheme.

Keywords: DHA; EPA; Fatty acid; Lipid; Omega; PUFA; Thraustochytrium sp..

MeSH terms

Docosahexaenoic Acids
Eicosapentaenoic Acid
Fatty Acids
Fatty Acids, Omega-3*
Fatty Acids, Unsaturated
Stramenopiles*

Substances

Fatty Acids, Unsaturated
Eicosapentaenoic Acid
Docosahexaenoic Acids
Fatty Acids, Omega-3
Fatty Acids