Methods for mapping 3D-chromosome architecture around nucleoli

Cristiana Bersaglieri; Raffaella Santoro

doi:10.1016/j.ceb.2023.102171

Methods for mapping 3D-chromosome architecture around nucleoli

Curr Opin Cell Biol. 2023 Apr:81:102171. doi: 10.1016/j.ceb.2023.102171. Epub 2023 May 23.

Authors

Cristiana Bersaglieri¹, Raffaella Santoro²

Affiliations

¹ Department of Molecular Mechanisms of Disease, DMMD, University of Zurich, Zurich, Switzerland.
² Department of Molecular Mechanisms of Disease, DMMD, University of Zurich, Zurich, Switzerland. Electronic address: raffaella.santoro@dmmd.uzh.ch.

PMID: 37230037
DOI: 10.1016/j.ceb.2023.102171

Abstract

The nucleolus is the largest subcompartment of the nucleus, known to be the place of ribosome biogenesis. Emerging evidence has started to implicate the nucleolus in the organization of chromosomes in the nucleus. Genomic domains contacting the nucleolus are defined as nucleolar associated domains (NADs) and are generally characterized by repressive chromatin states. However, the role of the nucleolus in genome architecture remains still not fully understood mainly because the lack of a membrane has challenged the establishment of methods for accurate identification of NADs. Here, we will discuss recent advances on methods to identify and characterize NADs, discuss their improvements relative to old methods, and highlight future perspectives.

Keywords: Genome organization; NADs; Nucleolus.

Publication types

Review

MeSH terms

Cell Nucleolus* / genetics
Cell Nucleus* / genetics
Chromatin / genetics
Genome

Substances

4-nitro-4'-aminodiphenyl sulfone
Chromatin