Molecular characterization and expression analysis of the remorin genes in tomato (Solanum lycopersicum L.)

Hui Li; Xiao Wang; Yue Zhuo; Shuisen Chen; Jingwei Lin; Hui Ma; Ming Zhong

doi:10.3389/fpls.2023.1175153

Molecular characterization and expression analysis of the remorin genes in tomato (Solanum lycopersicum L.)

Front Plant Sci. 2023 May 9:14:1175153. doi: 10.3389/fpls.2023.1175153. eCollection 2023.

Authors

Hui Li¹, Xiao Wang¹, Yue Zhuo¹, Shuisen Chen¹, Jingwei Lin¹, Hui Ma¹, Ming Zhong¹

Affiliation

¹ Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China.

Abstract

Remorin (REMs) are plant-specific and plasma membrane-associated proteins that play an essential role in the growth and development of plants and adaptations to adverse environments. To our knowledge, a genome-scale investigation of the REM genes in tomato has never been systematically studied. In this study, a total of 17 SlREM genes were identified in the tomato genome using bioinformatics methods. Our results demonstrated that the 17 members of SlREM were classified into 6 groups based on phylogenetic analysis and unevenly distributed on the eight chromosomes of tomato. There were 15 REM homologous gene pairs between tomato and Arabidopsis. The SlREM gene structures and motif compositions were similar. Promoter sequence analysis showed that the SlREM gene promoters contained some tissue-specific, hormones and stress-related cis-regulatory elements. Expression analysis based on qRT-PCR (Real-time quantitative PCR) analysis showed that SlREM family genes were were differentially expressed in different tissues, and they responded to ABA, MeJA, SA, low-temperature, drought and NaCl treatments. These results potentially provide relevant information for further research on the biological functions of SlREM family genes.

Keywords: expression pattern; gene family; genome-wide analysis; remorin; tomato.

Grants and funding

This work was supported by the National Natural Science Foundation of China (Grant No. 31070448) and the Liaoning Province ‘2021 Special Project of Central Government Guiding local scientific technology development’ (2021JH6/10500164). The funding bodies had no roles in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.