Transcriptional dynamics of delaminating neuroblasts in the mouse otic vesicle

Maggie S Matern; Robert Durruthy-Durruthy; Onur Birol; Spyros Darmanis; Mirko Scheibinger; Andrew K Groves; Stefan Heller

doi:10.1016/j.celrep.2023.112545

Transcriptional dynamics of delaminating neuroblasts in the mouse otic vesicle

Cell Rep. 2023 Jun 27;42(6):112545. doi: 10.1016/j.celrep.2023.112545. Epub 2023 May 23.

Authors

Maggie S Matern¹, Robert Durruthy-Durruthy¹, Onur Birol², Spyros Darmanis³, Mirko Scheibinger¹, Andrew K Groves⁴, Stefan Heller⁵

Affiliations

¹ Department of Otolaryngology Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA; Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA.
² Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.
³ Departments of Bioengineering and Applied Physics and Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.
⁴ Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
⁵ Department of Otolaryngology Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA; Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA. Electronic address: hellers@stanford.edu.

Abstract

An abundance of research has recently highlighted the susceptibility of cochleovestibular ganglion (CVG) neurons to noise damage and aging in the adult cochlea, resulting in hearing deficits. Furthering our understanding of the transcriptional cascades that contribute to CVG development may provide insight into how these cells can be regenerated to treat inner ear dysfunction. Here we perform a high-depth single-cell RNA sequencing analysis of the E10.5 otic vesicle and its surrounding tissues, including CVG precursor neuroblasts and emerging CVG neurons. Clustering and trajectory analysis of otic-lineage cells reveals otic markers and the changes in gene expression that occur from neuroblast delamination toward the development of the CVG. This dataset provides a valuable resource for further identifying the mechanisms associated with CVG development from neurosensory competent cells within the otic vesicle.

Keywords: CP: Neuroscience; cochlea; cochleovestibular ganglion; neurogenesis; otic neuroblasts; otic placode; otic vesicle; scRNA-seq; vestibular.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Animals
Cochlea*
Gene Expression Regulation, Developmental
Mice
Neural Stem Cells* / metabolism
Neurons

Abstract

Publication types

MeSH terms

Grants and funding