Laser-induced Fe3O4 nanoparticle-modified three-dimensional macroporous graphene is fabricated as a near-infrared light responsive nanozyme via a facile and in situ laser-scanning method under ambient conditions for the first time, showing an excellent catalytic-photothermal synergetic bactericidal ability under a low dose of H2O2 (0.1 mM) and short irradiation time (5.0 min).