Chronic D-galactose administration induces natural aging characteristics, in rat's brain and heart

Patcharapong Pantiya; Chanisa Thonusin; Benjamin Ongnok; Titikorn Chunchai; Aphisek Kongkaew; Wichwara Nawara; Busarin Arunsak; Nipon Chattipakorn; Siriporn C Chattipakorn

doi:10.1016/j.tox.2023.153553

Chronic D-galactose administration induces natural aging characteristics, in rat's brain and heart

Toxicology. 2023 Jun 15:492:153553. doi: 10.1016/j.tox.2023.153553. Epub 2023 May 22.

Authors

Patcharapong Pantiya¹, Chanisa Thonusin¹, Benjamin Ongnok¹, Titikorn Chunchai², Aphisek Kongkaew³, Wichwara Nawara², Busarin Arunsak², Nipon Chattipakorn¹, Siriporn C Chattipakorn⁴

Affiliations

¹ Neurophysiology Unit, Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Cardiac Electrophysiology Unit, Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand.
² Neurophysiology Unit, Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand.
³ Research Administration Section, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
⁴ Neurophysiology Unit, Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Cardiac Electrophysiology Unit, Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Department of Oral Biology and Diagnostic Sciences, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand. Electronic address: siriporn.c@cmu.ac.th.

PMID: 37225035
DOI: 10.1016/j.tox.2023.153553

Abstract

We aimed to investigate the effect of chronic D-galactose exposure on the mimicking of natural aging processes based upon the hallmarks of aging. Seven-week-old male Wistar rats (n = 12) were randomly assigned to receive either normal saline solution as a vehicle (n = 6) or 150 mg/kg/day of D-galactose subcutaneously for 28 weeks. Seventeen-month-old rats (n = 6) were also included as the chronologically aged controls. At the end of week 28 of the experiment (when the rats reach 35 weeks old and 24 months old), all rats were sacrificed for brain and heart collection. Our results showed that chronic D-galactose exposure mimicked natural aging characteristics of the brain and the heart in terms of deregulated nutrient sensing, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, altered intercellular communication, and functional impairment. All of which highlight the potential of D-galactose as a substance for inducing brain and cardiac aging in animal experiments.

Keywords: Aging; Cardiovascular disease; D-galactose; Hallmarks of aging; Natural aging; Neurodegenerative disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aging* / physiology
Animals
Brain
Cellular Senescence
Galactose* / toxicity
Male
Rats
Rats, Wistar

Substances

Galactose