Comparison of extruded cell nanovesicles and exosomes in their molecular cargos and regenerative potentials

Xianyun Wang; Shiqi Hu; Dashuai Zhu; Junlang Li; Ke Cheng; Gang Liu

doi:10.1007/s12274-023-5374-3

Comparison of extruded cell nanovesicles and exosomes in their molecular cargos and regenerative potentials

Nano Res. 2023;16(5):7248-7259. doi: 10.1007/s12274-023-5374-3. Epub 2023 Feb 28.

Authors

Xianyun Wang^{1

2

3

4

5

6}, Shiqi Hu^{5

6}, Dashuai Zhu^{5

6}, Junlang Li^{5

6}, Ke Cheng^{5

6}, Gang Liu^{1

3

4}

Affiliations

¹ Department of Cardiology, The First Hospital of Hebei Medical University, Shijiazhuang, 050000 China.
² Scientific Research Data Center, The First Hospital of Hebei Medical University, Shijiazhuang, 050000 China.
³ Hebei Key Laboratory of Cardiac Injury Repair Mechanism Study, Shijiazhuang, 050000 China.
⁴ Hebei International Joint Research Center for Structural Heart Disease, Shijiazhuang, 050000 China.
⁵ Department of Molecular Biomedical Science, North Carolina State University, Raleigh, North Carolina 27607 USA.
⁶ Department of Biomedical Engineering, University of North Carolina, Chapel Hill and North Carolina State University, Raleigh, North Carolina 27607 USA.

Abstract

Extracellular vesicles (EVs) generated from mesenchymal stem cells (MSCs) play an essential role in modulating cell-cell communication and tissue regeneration. The clinical translation of EVs is constrained by the poor yield of EVs. Extrusion has recently become an effective technique for producing a large scale of nanovesicles (NVs). In this study, we systematically compared MSC NVs (from extrusion) and EVs (from natural secretion). Proteomics and RNA sequencing data revealed that NVs resemble MSCs more closely than EVs. Additionally, microRNAs in NVs are related to cardiac repair, fibrosis repression, and angiogenesis. Lastly, intravenous delivery of MSC NVs improved heart repair and cardiac function in a mouse model of myocardial infarction.

Electronic supplementary material: Supplementary material (Figs. S1-S4) is available in the online version of this article at 10.1007/s12274-023-5374-3.

Keywords: cardiac repair; exosomes; extracellular vesicles (EVs); mesenchymal stem cells (MSCs); myocardial infarction (MI); nanovesicles (NVs).