In this paper, the effect of the laser repetition rate on the long-distance femtosecond laser filament in air is investigated by measuring the fluorescence characteristic of the filament. A femtosecond laser filament emits fluorescence due to the thermodynamical relaxation of the plasma channel. Experimental results show that as the repetition rate of femtosecond laser increases, the fluorescence of the filament induced by a single laser pulse weakens, and the position of the filament moves away from the focusing lens. These phenomena may be attributed to the slow hydrodynamical recovery process of air after being excited by a femtosecond laser filament, whose characteristic time is on the millisecond time scale and comparable to the inter-pulse duration of the femtosecond laser pulse train. This finding suggests that at a high laser repetition rate, to generate an intense laser filament, the femtosecond laser beam should scan across the air to eliminate the adverse effect of slow air relaxation, which is beneficial to laser filament remote sensing.