The insatiable growth of datacenter traffic mandates increasing the capacity of cost-effective intensity modulation direct detection (IMDD) systems to meet the foreseen demand. This Letter demonstrates the first, to the best of our knowledge, single-digital-to-analog converter (DAC) IMDD system achieving a net 400-Gbps transmission using a thin-film lithium niobate (TFLN) Mach-Zehnder modulator (MZM). Employing a driver-less DAC channel (128 GSa/s, 800 mVpp) with neither pulse-shaping nor pre-emphasis filtering, we transmit (1) 128-Gbaud PAM16 below the 25% overhead soft-decision forward error correction (SD-FEC) bit error rate (BER) threshold and (2) 128-Gbaud probabilistically shaped (PS)-PAM16 under the 20% overhead SD-FEC threshold, which respectively correspond to record net rates of 410 and 400 Gbps for single-DAC operation. Our results highlight the promise of operating 400-Gbps IMDD links with reduced digital signal processing (DSP) complexity and driving swing requirements.