Phylogenics of the genus Glossogobius in the Mekong Delta based on the mitochondrial cytochrome b (cytb) gene

Gieo Hoang Phan; Tran Thi Huyen Lam; Quang Minh Dinh; Ton Huu Duc Nguyen

doi:10.1016/j.heliyon.2023.e16106

Phylogenics of the genus Glossogobius in the Mekong Delta based on the mitochondrial cytochrome b (cytb) gene

Heliyon. 2023 May 10;9(5):e16106. doi: 10.1016/j.heliyon.2023.e16106. eCollection 2023 May.

Authors

Gieo Hoang Phan^{1

2}, Tran Thi Huyen Lam^{1

3}, Quang Minh Dinh⁴, Ton Huu Duc Nguyen⁴

Affiliations

¹ Department of Molecular Biotechnology, Institute of Food and Biotechnology, Can Tho University, Xuan Khanh Ward, Ninh Kieu District, Can Tho, 900000, Viet Nam.
² Kien Giang University, Minh Luong Town, Chau Thanh District, Kien Giang, 920000, Viet Nam.
³ Institute of High Quality Biotechnology - Food Technology, Cuu Long University, National Road 1A, Phu Quoi Ward, Long Ho District, Vinh Long, 850000, Viet Nam.
⁴ Department of Biology, School of Education, Can Tho University, Xuan Khanh Ward, Ninh Kieu District, Can Tho, 900000, Viet Nam.

Abstract

The Glossogobius species play an essential role in food supply and are distributed widely from the marine to freshwater, especially in the Mekong Delta, Vietnam (VMD). Some of their morphometrics and meristics are observed to vary with species and sampling sites. Therefore, the present study aims to verify if their mitochondrial Cytochrome b (Cytb) gene, one of the popular gene sequences used in fish phylogenic variation assessment, varies with species and sampling sites in the VMD. The Cytb gene size was 1300 bp for GcytbH/GcytbL primer pair and 1045 bp for GluMuq1-F/Mixcyto937-2R. The genetic distances within and among these three fish species groups were 0-11%. The Cytb gene sequences' similarity between this study and the NCBI database was 85.84-100%. The Glossogobius specimens were observed to disperse in small branches of the phylogenetic tree with a low K2P value, suggesting that the Cytb genetic diversity may be low among species.

Keywords: Cytb; Genetic distance; Glossogobius aureus; Glossogobius giuris; Glossogobius sparsipapillus; Phylogeny.