Purification and identification of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes

Liangnan Cui; Jing Wu; Xiang Wang; Xiaotong Yang; Zixin Ye; Kevin H Mayo; Lin Sun; Yifa Zhou

doi:10.1016/j.fochx.2023.100706

Purification and identification of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes

Food Chem X. 2023 May 6:18:100706. doi: 10.1016/j.fochx.2023.100706. eCollection 2023 Jun 30.

Authors

Liangnan Cui¹, Jing Wu¹, Xiang Wang¹, Xiaotong Yang¹, Zixin Ye¹, Kevin H Mayo², Lin Sun¹, Yifa Zhou¹

Affiliations

¹ Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, China.
² Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 6-155 Jackson Hall, Minneapolis, MN 55455, USA.

Abstract

Even though Cimicifuga sp. is widely used in functional foods around the world, the content and structure of its oligosaccharides remain unclear. Here, we isolated a mixture of oligosaccharides from Cimicifuga heracleifolia Kom. rhizomes with a yield of 9.5% w/w. Twenty-six oligosaccharide monomers from the mixture were purified using optimized SEC and HILIC techniques. The oligosaccharides were identified as belonging to two groups by using HPAEC-PAD, MALDI-TOF-MS, NMR and GC-MS methylation analyses. One group belongs to sucrose and inulin type fructo-oligosaccharides (FOS) {β-d-Fruf-(2 → 1)-[β-d-Fruf-(2 $\leftrightarrow$ 1)]_n=1-12-α-d-Glcp} with a 3-14 degree of polymerization (DP). Oligosaccharides in the other group belong to the inulo-n-ose type FOS {β-d-Fruf-(2 → 1)-[β-d-Fruf-(2 → 1)]_m=0-12-β-d-Frup} with a DP of 2-14. This appears to be the first time that these oligosaccharides have been purified from Cimicifuga heracleifolia Kom., thus providing useful information concerning the utilization of Cimicifuga heracleifolia Kom. in functional foods.

Keywords: Cimicifuga heracleifolia Kom.; Fructo-oligosaccharides; Purification; Structural elucidation.