Biochemical, structural characterization and in-vitro evaluation of antioxidant, antibacterial, cytotoxic, and antidiabetic activities of nanosuspensions of Cinnamomum zeylanicum bark extract

Aqsa Nawaz; Tayyab Ali; Muhammad Naeem; Fatma Hussain; Zhiye Li; Abdul Nasir

doi:10.3389/fchem.2023.1194389

Biochemical, structural characterization and in-vitro evaluation of antioxidant, antibacterial, cytotoxic, and antidiabetic activities of nanosuspensions of Cinnamomum zeylanicum bark extract

Front Chem. 2023 May 5:11:1194389. doi: 10.3389/fchem.2023.1194389. eCollection 2023.

Authors

Aqsa Nawaz¹, Tayyab Ali¹, Muhammad Naeem², Fatma Hussain¹, Zhiye Li³, Abdul Nasir⁴

Affiliations

¹ Clinico-Molecular Biochemistry Laboratory, Department of Biochemistry, Faculty of Sciences, University of Agriculture, Faisalabad, Pakistan.
² College of Life Science, Hebei Normal University, Shijiazhuang, China.
³ Department of Pharmacy, Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
⁴ Medical Research Center, Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Abstract

Cinnamomum zeylanicum is a traditional medicinal plant known for its anti-inflammatory, antidiabetic, antimicrobial, anticancer, and antioxidant properties. Its therapeutic efficacy using nanosuspensions is still unclear for treating infectious diseases. This study was designed to evaluate the bioactivities, biochemical characterization, and bioavailability of freshly prepared nanosuspensions of C. zeylanicum. Structural and biochemical characterization of C. zeylanicum and its biological activities, such as antioxidants, antimicrobials, antiglycation, α-amylase inhibition, and cytotoxicity was performed using Fourier-transform infrared (FTIR) spectroscopy and High-Performance Liquid Chromatography (HPLC). C. zeylanicum extract and nanosuspensions showed TPCs values of 341.88 and 39.51 mg GAE/100 g while showing TFCs as 429.19 and 239.26 mg CE/100g, respectively. DPPH inhibition potential of C. zeylanicum extract and nanosuspension was 27.3% and 10.6%, respectively. Biofilm inhibition activity revealed that bark extract and nanosuspension showed excessive growth restraint against Escherichia coli, reaching 67.11% and 66.09%, respectively. The α-amylase inhibition assay of extract and nanosuspension was 39.3% and 6.3%, while the antiglycation activity of nanosuspension and extract was 42.14% and 53.76%, respectively. Extracts and nanosuspensions showed maximum hemolysis at 54.78% and 19.89%, respectively. Results indicated that nanosuspensions possessed antidiabetic, antimicrobial, anticancer, and antioxidant properties. Further study, however, is needed to assess the clinical studies for the therapeutic use of nanosuspensions.

Keywords: alpha-amylase inhibition; antidiabetic potential; antioxidant potential; nanosuspensions; therapeutic efficacy.

Grants and funding

This study was financially supported by grants from 2021 Young and middle-aged academic leaders of health in Henan Province, (No: HNSWJW-2021001); Program for Science and Technology Innovation Talents in Universities of Henan Province (No: 22HASTIT047).