Genus Paris has numerous bioactive constituents such as steroid saponins, flavonoids, and polysaccharose which are responsible for antitumor, hemostatic, and anthelmintic, etc. In this study, ultrahigh performance liquid chromatography coupled to time-of-flight mass spectrometer (UHPLC-QTOF-MS) and Fourier transform infrared (FT-IR) spectroscopy in combination with multivariable analysis were employed to discriminate the different species of Paris including P. polyphylla var. yunnanensis (PPY), P. polyphylla var. alba, P. mairei (PM), P. vietnamensis, and P. polyphylla var. stenophylla. Partial least square discriminate analysis based on UHPLC, FT-IR, and midlevel data fusion was used to distinguish 43 batches of Paris. Chemical constituents of different species Paris were determined by UHPLC-QTOF-MS. The result indicated that midlevel data fusion had a good performance in the classification compared to a single analytical technology. A total of 47 compounds were identified in different species Paris. The similar results indicated that PM could be treated as a proposal substitute of PPY.
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