Clusters of nearly identical water microdroplets levitating over a locally heated water layer are considered. The high-resolution and high-speed fluorescence microscopy showed that there is a universal brightness profile of single droplets, and this profile does not depend on the droplet temperature and size. We explain this universal profile using the theory of light scattering and propose a new method for determining the parameters of possible optical inhomogeneity of a droplet from its fluorescent image. In particular, we report for the first time and explain the anomalous fluorescence of some large droplets with initially high brightness at the periphery of the droplet. The disappearance of this effect after a few seconds is related to the diffusion of the fluorescent substance in water. Understanding the fluorescence profiles paves the way for the application of droplet clusters to the laboratory study of biochemical processes in individual microdroplets.