Generation and characterization of a monoclonal antibody that binds to Galectin-1

Kirsten Pfeffer; Thai H Ho; Francisca J Grill; Yvette Ruiz; Douglas F Lake

doi:10.1016/j.pep.2023.106308

Generation and characterization of a monoclonal antibody that binds to Galectin-1

Protein Expr Purif. 2023 Oct:210:106308. doi: 10.1016/j.pep.2023.106308. Epub 2023 May 18.

Authors

Kirsten Pfeffer¹, Thai H Ho², Francisca J Grill¹, Yvette Ruiz¹, Douglas F Lake³

Affiliations

¹ School of Life Sciences, Arizona State University, Phoenix, AZ, USA.
² Division of Hematology/Oncology, Mayo Clinic Arizona, Phoenix, AZ, USA.
³ School of Life Sciences, Arizona State University, Phoenix, AZ, USA. Electronic address: Douglas.Lake@asu.edu.

PMID: 37209931
PMCID: PMC10330795 (available on 2024-10-01)
DOI: 10.1016/j.pep.2023.106308

Abstract

Galectin-1 is a β-galactoside-binding lectin that has been implicated as a suppressive molecule in cancer and autoimmune diseases. Gal-1 has known immunomodulatory activity and was found to be expressed on regulatory T cells, leading to the potential for targeted immunotherapies. Anti-Gal-1 monoclonal antibodies were generated in this study using classical hybridoma techniques. MAb 6F3 was found to bind to Gal-1 by Western blot and ELISA. Flow cytometry was used to determine cell surface and intracellular binding of mAb 6F3 to Gal-1 in PBMC-derived Tregs and tumor cells, including Treg-like cell lines. These results suggest mAb 6F3 may be used to further study Gal-1 protein expression and function.

Keywords: Galectin-1; Immune suppression; Monoclonal antibody.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Antibodies, Monoclonal* / metabolism
Enzyme-Linked Immunosorbent Assay
Galectin 1* / metabolism
Leukocytes, Mononuclear / metabolism
T-Lymphocytes, Regulatory / metabolism

Substances

Galectin 1
Antibodies, Monoclonal

Abstract

Publication types

MeSH terms

Substances

Grants and funding