Fermentative valorisation of xylose-rich hemicellulosic hydrolysates from agricultural waste residues for lactic acid production under non-sterile conditions

Rylan Cox; Vivek Narisetty; Eulogio Castro; Deepti Agrawal; Samuel Jacob; Gopalakrishnan Kumar; Deepak Kumar; Vinod Kumar

doi:10.1016/j.wasman.2023.05.015

Fermentative valorisation of xylose-rich hemicellulosic hydrolysates from agricultural waste residues for lactic acid production under non-sterile conditions

Waste Manag. 2023 Jul 1:166:336-345. doi: 10.1016/j.wasman.2023.05.015. Epub 2023 May 18.

Authors

Rylan Cox¹, Vivek Narisetty², Eulogio Castro³, Deepti Agrawal⁴, Samuel Jacob⁵, Gopalakrishnan Kumar⁶, Deepak Kumar⁷, Vinod Kumar⁸

Affiliations

¹ School of Aerospace, Transport and Manufacturing, Cranfield University, Cranfield MK43 0AL, UK.
² School of Water, Energy and Environment, Cranfield University, Cranfield MK43 0AL, UK.
³ Department of Chemical, Environmental and Materials Engineering, Universidad de Jaén, Campus LasLagunillas, 23071 Jaén, Spain.
⁴ Biochemistry and Biotechnology Area, Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun 248005, India.
⁵ Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu 603203, India.
⁶ School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea.
⁷ Department of Chemical Engineering, SUNY College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
⁸ School of Water, Energy and Environment, Cranfield University, Cranfield MK43 0AL, UK; Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India. Electronic address: Vinod.Kumar@cranfield.ac.uk.

PMID: 37209430
DOI: 10.1016/j.wasman.2023.05.015

Abstract

Lactic acid (LA) is a platform chemical with diverse industrial applications. Presently, commercial production of LA is dominated by microbial fermentation using sugary or starch-based feedstocks. Research pursuits emphasizing towards sustainable production of LA using non-edible and renewable feedstocks have accelerated the use of lignocellulosic biomass (LCB). The present study focuses on the valorisation of xylose derived from sugarcane bagasse (SCB) and olive pits (OP) through hydrothermal and dilute acid pretreatment, respectively. The xylose-rich hydrolysate obtained was used for LA production by homo-fermentative and thermophilic Bacillus coagulans DSM2314 strain under non-sterile conditions. The fed-batch mode of fermentation resulted in maximum LA titers of 97.8, 52.4 and 61.3 g/L with a yield of 0.77, 0.66 and 0.71 g/g using pure xylose, xylose-rich SCB and OP hydrolysates, respectively. Further, a two-step aqueous two-phase system (ATPS) extraction technique was employed for the separation and recovery of LA accumulated on pure and crude xylose. The LA recovery was 45 - 65% in the first step and enhanced to 80-90% in the second step.The study demonstrated an efficient integrated biorefinery approach to valorising the xylose-rich stream for cost-effective LA production and recovery.

Keywords: Bacillus coagulans; Lactic acid; Non-sterile conditions; Olive pits; Sugarcane bagasse; Xylose-rich hydrolysate.

MeSH terms

Cellulose*
Fermentation
Lactic Acid
Saccharum*
Xylose

Substances

Cellulose
Xylose
Lactic Acid