Correlative Fluorescent Scanning Probe Nanotomography Used to Study the Intracellular Distribution of Doxorubicin in MCF-7 Human Breast Adenocarcinoma Cells

O I Agapova; A E Efimov; K E Mochalov; D O Solovyeva; A M Gileva; E A Markvicheva; D V Yakovlev; A V Lyundup; V A Oleinikov; I I Agapov; S V Gautier

doi:10.1134/S0012496623700266

Correlative Fluorescent Scanning Probe Nanotomography Used to Study the Intracellular Distribution of Doxorubicin in MCF-7 Human Breast Adenocarcinoma Cells

Dokl Biol Sci. 2023 Apr;509(1):103-106. doi: 10.1134/S0012496623700266. Epub 2023 May 19.

Authors

O I Agapova¹, A E Efimov², K E Mochalov³, D O Solovyeva³, A M Gileva³, E A Markvicheva³, D V Yakovlev³, A V Lyundup⁴, V A Oleinikov^{3

5}, I I Agapov², S V Gautier^{2

6}

Affiliations

¹ Shumakov National Medical Research Center of Transplantology and Artificial Organs, Moscow, Russia. igor_agapov@mail.ru.
² Shumakov National Medical Research Center of Transplantology and Artificial Organs, Moscow, Russia.
³ Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
⁴ Рeoples' Friendship University of Russia, Moscow, Russia.
⁵ National Research Nuclear University, Moscow, Russia.
⁶ Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.

PMID: 37208575
DOI: 10.1134/S0012496623700266

Abstract

Developing technologies for efficient targeted drug delivery for oncotherapy requires new methods to analyze the features of micro- and nanoscale distributions of antitumor drugs in cells and tissues. A new approach to three-dimensional analysis of the intracellular distribution of cytostatics was developed using fluorescence scanning optical-probe nanotomography. A correlative analysis of the nanostructure and distribution of injected doxorubicin in MCF-7 human breast adenocarcinoma cells revealed the features of drug penetration and accumulation in the cell. The technology is based on the principles of scanning optical probe nanotomography and is applicable to studying the distribution patterns of various fluorescent or fluorescence-labelled substances in cells and tissues.

Keywords: MCF-7 human adenocarcinoma cells; cytostatics; fluorescence microscopy; nanotomography; scanning probe microscopy.

MeSH terms

Adenocarcinoma* / drug therapy
Breast Neoplasms* / drug therapy
Breast Neoplasms* / pathology
Doxorubicin / pharmacology
Female
Fluorescent Dyes
Humans
MCF-7 Cells

Substances

Fluorescent Dyes
Doxorubicin