WIPI2 enhances the vulnerability of colorectal cancer cells to erastin via bioinformatics analysis and experimental verification

Liying Yu; Yan Luo; Xile Ding; Miaomiao Tang; Huan Gao; Renfang Zhang; Mingfu Chen; Yuchen Liu; Qiongxia Chen; Yanli Ouyang; Xiang Wang; Hongyan Zhen

doi:10.3389/fonc.2023.1146617

WIPI2 enhances the vulnerability of colorectal cancer cells to erastin via bioinformatics analysis and experimental verification

Front Oncol. 2023 May 3:13:1146617. doi: 10.3389/fonc.2023.1146617. eCollection 2023.

Authors

Liying Yu¹, Yan Luo¹, Xile Ding¹, Miaomiao Tang¹, Huan Gao¹, Renfang Zhang¹, Mingfu Chen¹, Yuchen Liu^{2

3}, Qiongxia Chen¹, Yanli Ouyang⁴, Xiang Wang⁵, Hongyan Zhen^{1

2}

Affiliations

¹ Department of Pathology and Pathophysiology, School of Medicine, Jianghan University, Wuhan, China.
² Cancer Institute, School of Medicine, Jianghan University, Wuhan, China.
³ Wuhan Institute of Biomedical Sciences, School of Medicine, Jianghan University, Wuhan, China.
⁴ Department of Pathology, Fifth Hospital in Wuhan, Wuhan, China.
⁵ Department of Histology and Embryology, School of Medicine, Jianghan University, Wuhan, China.

Abstract

Introduction: WD Repeat Domain Phosphoinositide Interacting 2 (WIPI2) is a WD repeat protein that interacts with phosphatidylinositol and regulates multiprotein complexes by providing a b-propeller platform for synchronous and reversible protein-protein interactions assembled proteins. Ferroptosis is a novel iron-dependent form of cell death. It is usually accompanied with the accumulation of membrane lipid peroxides. Our study is to focus on investigating the effect of WIPI2 on the growth and ferroptosis of colorectal cancer (CRC) cells and its potential mechanism.

Methods: We analyzed the expression of WIPI2 in colorectal cancer versus normal tissues through The Cancer Genome Atlas (TCGA), and the relationship between clinical traits and WIPI2 expression and prognosis was assessed by univariate and multifactorial cox analysis. Next, we constructed the siRNAs targeting the WIPI2 sequence si-WIPI2 to further investigate the mechanism of WIPI2 in CRC cells through vitro experiments.

Results: Public data from the TCGA platform showed that WIPI2 expression was significantly elevated in colorectal cancer tissues compared to paracancerous tissues, and high WIPI2 expressionpredicted poor prognosis for CRC patients. Moreover, we found that the knockdown of WIPI2 expression could inhibit the growth and proliferation of HCT116 and HT29 cells. Furthermore, we found that the expression level of ACSL4 decreased and that of GPX4 increased when WIPI2 was knocked down, suggesting that WIPI2 can potentially positively regulate CRC ferroptosis. Meanwhile, both NC and si groups were able to further inhibit cell growth activity, as well as increase WIPI2 and decrease GPX4 expression when treated with Erastin, but the rate of cell viability inhibition and the trend of protein changes were more significantly in the NC group than si groups, which indicated that Erastin induced CRC ferroptosis through the WIPI2/GPX4 pathway thereby enhancing the sensitivity of colorectal cancer cells to Erastin.

Conclusions: Our study suggested that WIPI2 had a promotional effect on the growth of colorectal cancer cells, and it also played an important role in the ferroptosis pathway.

Keywords: WIPI2; colorectal cancer; erastin; ferroptosis; glutathione peroxidase 4.