Detection speed optimization of the OI-RD microscope for ultra-high throughput screening

Hang Zhang; Mengjing Xu; Haofeng Li; Xiaohan Mai; Jiawei Sun; Lan Mi; Jiong Ma; Xiangdong Zhu; Yiyan Fei

doi:10.1364/BOE.487563

Detection speed optimization of the OI-RD microscope for ultra-high throughput screening

Biomed Opt Express. 2023 Apr 28;14(5):2386-2399. doi: 10.1364/BOE.487563. eCollection 2023 May 1.

Authors

Hang Zhang¹, Mengjing Xu¹, Haofeng Li¹, Xiaohan Mai¹, Jiawei Sun², Lan Mi¹, Jiong Ma¹, Xiangdong Zhu³, Yiyan Fei¹

Affiliations

¹ Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai, 200433, China.
² Department of Science and Technology, Shanghai Deyu Intelligent Technology Co., Ltd., Shanghai, 201413, China.
³ Department of Physics, University of California, One Shields Avenue, Davis, California 95616, USA.

Abstract

The oblique-incidence reflectivity difference (OI-RD) microscope is a label-free detection system for microarrays that has many successful applications in high throughput drug screening. The increase and optimization of the detection speed of the OI-RD microscope will enable it to be a potential ultra-high throughput screening tool. This work presents a series of optimization methods that can significantly reduce the time to scan an OI-RD image. The wait time for the lock-in amplifier was decreased by the proper selection of the time constant and development of a new electronic amplifier. In addition, the time for the software to acquire data and for translation stage movement was also minimized. As a result, the detection speed of the OI-RD microscope is 10 times faster than before, making the OI-RD microscope suitable for ultra-high throughput screening applications.