Ionic displacement of Ca2+ by Pb2+ in calmodulin is affected by arrhythmia-associated mutations

Abstract

Lead is a highly toxic metal that severely perturbs physiological processes even at sub-micromolar levels, often by disrupting the Ca²⁺ signaling pathways. Recently, Pb²⁺-associated cardiac toxicity has emerged, with potential involvement of both the ubiquitous Ca²⁺ sensor protein calmodulin (CaM) and ryanodine receptors. In this work, we explored the hypothesis that Pb²⁺ contributes to the pathological phenotype of CaM variants associated with congenital arrhythmias. We performed a thorough spectroscopic and computational characterization of CaM conformational switches in the co-presence of Pb²⁺ and four missense mutations associated with congenital arrhythmias, namely N53I, N97S, E104A and F141L, and analyzed their effects on the recognition of a target peptide of RyR2. When bound to any of the CaM variants, Pb²⁺ is difficult to displace even under equimolar Ca²⁺ concentrations, thus locking all CaM variants in a specific conformation, which exhibits characteristics of coiled-coil assemblies. All arrhythmia-associated variants appear to be more susceptible to Pb²⁺ than wild type (WT) CaM, as the conformational transition towards the coiled-coil conformation occurs at lower Pb²⁺, regardless of the presence of Ca²⁺, with altered cooperativity. The presence of arrhythmia-associated mutations specifically alters the cation coordination of CaM variants, in some cases involving allosteric communication between the EF-hands in the two domains. Finally, while WT CaM increases the affinity for the RyR2 target in the presence of Pb²⁺, no specific pattern could be detected for all other variants, ruling out a synergistic effect of Pb²⁺ and mutations in the recognition process.

Keywords: Arrhythmia; Ca(2+)-sensor; Calmodulin; Circular dichroism; Heavy metals; Ionic displacement; Lead; Molecular dynamics; Ryanodine receptors.