Dual-mode immunochromatographic assay based on dendritic gold nanoparticles with superior fluorescence quenching for ultrasensitive detection of E. coli O157:H7

Yanna Shao; Zhengzheng Wang; Jihang Xie; Zhenjun Zhu; Ying Feng; Shubo Yu; Liang Xue; Shi Wu; Qihui Gu; Jumei Zhang; Qingping Wu; Juan Wang; Yu Ding

doi:10.1016/j.foodchem.2023.136366

Dual-mode immunochromatographic assay based on dendritic gold nanoparticles with superior fluorescence quenching for ultrasensitive detection of E. coli O157:H7

Food Chem. 2023 Oct 30:424:136366. doi: 10.1016/j.foodchem.2023.136366. Epub 2023 May 13.

Authors

Yanna Shao¹, Zhengzheng Wang¹, Jihang Xie¹, Zhenjun Zhu², Ying Feng², Shubo Yu³, Liang Xue³, Shi Wu³, Qihui Gu³, Jumei Zhang³, Qingping Wu³, Juan Wang⁴, Yu Ding⁵

Affiliations

¹ Department of Food Science and Engineering, Institute of Food Safety and Nutrition, College of Science and Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China; Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
² Department of Food Science and Engineering, Institute of Food Safety and Nutrition, College of Science and Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
³ Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
⁴ College of Food Science, South China Agricultural University, Guangzhou 510432, China.
⁵ Department of Food Science and Engineering, Institute of Food Safety and Nutrition, College of Science and Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address: dingyu@jnu.edu.cn.

PMID: 37201472
DOI: 10.1016/j.foodchem.2023.136366

Abstract

We presented a colorimetric/fluorescent dual-mode immunochromatographic assay (ICA) for the sensitive detection of Escherichia coli O157:H7. The use of polydopamine (PDA)-modified gold nanoparticles (AuNPs) with broadband absorption allowed for excellent colorimetry signals for the ICA detection. Moreover, the absorption spectrum of PDA-AuNPs significantly overlaps with the excitation and emission spectra of ZnCdSe/ZnS quantum dots (QDs), resulting in effective quenching of the QDs fluorescence due to the inner filter effect. The fluorescence intensity changes induced by PDA-AuNPs were utilized for the sensitive detection of E. coli O157:H7, achieving a detection limit of 9.06 × 10¹ CFU/mL, which was 46-fold lower than that of traditional AuNPs-based immunoassay. The proposed immunosensor exhibited the recovery rate between 80.12% and 114.69% in detecting actual samples, indicating its reliability and satisfactory accuracy. This study provides insights into dual-mode signal outputs and the ICA development for food safety applications.

Keywords: Black nanoparticles; Branched nanostructures; Dual-mode signal; Escherichia coli O157:H7; Immunochromatographic assay; Inner filter effect.

MeSH terms

Biosensing Techniques* / methods
Escherichia coli O157*
Gold / chemistry
Immunoassay / methods
Metal Nanoparticles* / chemistry
Reproducibility of Results

Substances

Gold