Dual protective effect of the association of plant extracts and fluoride against dentine erosion: In the presence and absence of salivary pellicle

Samira Helena Niemeyer; Nikola Jovanovic; Sindy Sezer; Lucas Sébastien Wittwer; Tommy Baumann; Thiago Saads Carvalho

doi:10.1371/journal.pone.0285931

Dual protective effect of the association of plant extracts and fluoride against dentine erosion: In the presence and absence of salivary pellicle

PLoS One. 2023 May 18;18(5):e0285931. doi: 10.1371/journal.pone.0285931. eCollection 2023.

Authors

Samira Helena Niemeyer¹, Nikola Jovanovic¹, Sindy Sezer¹, Lucas Sébastien Wittwer¹, Tommy Baumann¹, Thiago Saads Carvalho¹

Affiliation

¹ Department of Restorative, Preventive and Pediatric Dentistry, University of Bern, Bern, Switzerland.

Abstract

Objectives: To verify the protective effect of plant extracts associated with fluoride against dental erosion of dentine, in the presence and absence of a salivary pellicle.

Methods: Dentine specimens (n = 270) were randomly distributed into 9 experimental groups (n = 30/group): GT (green tea extract); BE (blueberry extract); GSE (grape seed extract); NaF (sodium fluoride); GT+NaF (green tea extract and NaF); BE+NaF (blueberry extract and NaF); GSE+NaF (grape seed extract and NaF); negative control (deionized water); and a positive control (commercialized mouthrinse containing stannous and fluoride). Each group was further divided into two subgroups (n = 15), according to the presence (P) or absence (NP) of salivary pellicle. The specimens were submitted to 10 cycles: 30 min incubation in human saliva (P) or only in humid chamber (NP), 2 min immersion in experimental solutions, 60 min of incubation in saliva (P) or not (NP), and 1 min erosive challenge. Dentine surface loss (dSL-10 and dSL-total), amount of degraded collagen (dColl) and total calcium release (CaR) were evaluated. Data were analyzed with Kruskal-Wallis, Dunn's and Mann-Whitney U tests (p>0.05).

Results: Overall, the negative control presented the highest values of dSL, dColl and CaR, and the plant extracts showed different degrees of dentine protection. For the subgroup NP, GSE showed the best protection of the extracts, and the presence of fluoride generally further improved the protection for all extracts. For the subgroup P, only BE provided protection, while the presence of fluoride had no impact on dSL and dColl, but lowered CaR. The protection of the positive control was more evident on CaR than on dColl.

Conclusion: We can conclude that the plant extracts showed a protective effect against dentine erosion, regardless of the presence of salivary pellicle, and that the fluoride seems to improve their protection.

Copyright: © 2023 Niemeyer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Publication types

Randomized Controlled Trial
Research Support, Non-U.S. Gov't

MeSH terms

Dental Pellicle
Dentin
Fluorides / pharmacology
Grape Seed Extract* / pharmacology
Humans
Sodium Fluoride / pharmacology
Tea
Tooth Erosion* / prevention & control

Substances

Fluorides
Grape Seed Extract
Sodium Fluoride
Tea

Grants and funding

This study was supported/ funded by a grant from the German Society of Preventive (DGPZM) and CP GABA. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.