Comparative Analysis of the Therapeutic Effects of Fresh and Cryopreserved Human Umbilical Cord Derived Mesenchymal Stem Cells in the Treatment of Psoriasis

Zhifeng Wang; Yifan Hu; Xiaoyu Wang; Youdong Chen; Danfeng Wu; Houli Ji; Cuicui Yu; Jingmeng Fang; Chunrong Pan; Lianjian Wang; Shouxin Wang; Yinhong Guo; Yi Lu; Di Wu; Fangfang Ren; Hao Zhu; Yuling Shi

doi:10.1007/s12015-023-10556-8

Comparative Analysis of the Therapeutic Effects of Fresh and Cryopreserved Human Umbilical Cord Derived Mesenchymal Stem Cells in the Treatment of Psoriasis

Stem Cell Rev Rep. 2023 Aug;19(6):1922-1936. doi: 10.1007/s12015-023-10556-8. Epub 2023 May 18.

Authors

Zhifeng Wang^#^{1

2}, Yifan Hu^#^{3

4}, Xiaoyu Wang⁵, Youdong Chen^{3

4}, Danfeng Wu⁵, Houli Ji⁵, Cuicui Yu⁵, Jingmeng Fang⁵, Chunrong Pan⁵, Lianjian Wang⁵, Shouxin Wang⁵, Yinhong Guo⁵, Yi Lu⁵, Di Wu⁵, Fangfang Ren⁵, Hao Zhu⁶, Yuling Shi^{7

8}

Affiliations

¹ Department of Dermatology, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, 200443, China. wangzhifeng68@hotmail.com.
² Sinoneural Cell Engineering Group Holdings Co., Ltd, No. 1188, Lianhang Road, Shanghai, 201100, China. wangzhifeng68@hotmail.com.
³ Department of Dermatology, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, 200443, China.
⁴ Institute of Psoriasis, Tongji University School of Medicine, Shanghai, 200443, China.
⁵ Sinoneural Cell Engineering Group Holdings Co., Ltd, No. 1188, Lianhang Road, Shanghai, 201100, China.
⁶ Sinoneural Cell Engineering Group Holdings Co., Ltd, No. 1188, Lianhang Road, Shanghai, 201100, China. zhuhao@sinoneural.com.
⁷ Department of Dermatology, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, 200443, China. shiyuling1973@tongji.edu.cn.
⁸ Institute of Psoriasis, Tongji University School of Medicine, Shanghai, 200443, China. shiyuling1973@tongji.edu.cn.

^# Contributed equally.

PMID: 37199874
DOI: 10.1007/s12015-023-10556-8

Abstract

Psoriasis, an inflammatory autoimmune skin disease, is characterized by scaly white or erythematous plaques, which severely influence patients' quality of life and social activities. Mesenchymal stem cells derived from the human umbilical cord (UCMSCs) represent a promising therapeutic approach for psoriasis because of its unique superiority in ethical agreeableness, abundant source, high proliferation capacity, and immunosuppression. Although cryopreservation provided multiple benefits to the cell therapy, it also greatly compromised clinical benefits of MSCs due to impaired cell functions. The current study aims to evaluate the therapeutic efficacy of cryopreserved UCMSCs in a mouse model of psoriasis as well as in patients with psoriasis. Our results showed that cryopreserved and fresh UCMSCs have comparable effects on the suppression of psoriasis-like symptoms such as thickening, erythema, and scaling, and serum IL-17 A secretion in mice model of psoriasis. Moreover, psoriatic patients injected with cryopreserved UCMSCs had a significant improvement in the Psoriasis Area and Severity Index (PASI), Physician Global Assessment (PGA), and Patient Global Assessments (PtGAs) scores compared to baseline values. Mechanically, cryopreserved UCMSCs markedly inhibit the proliferation of PHA-activated PBMCs, type 1 T helper (Th1) and type 17 T helper (Th17) cell differentiation and secretion of inflammatory cytokines including IFN-γ, TNF-a and IL-17 A in PBMCs stimulated by anti-CD3/CD28 beads. Taken together, these data indicated that cryopreserved UCMSCs exhibited great beneficial effect on psoriasis. Thus, cryopreserved UCMSCs can be systemically administered as ''off-the-shelf'' cell product for psoriasis therapy. Trial Registration ChiCTR1800019509. Registered on November 15, 2018-Retrospectively registered, http://www.chictr.org.cn/ .

Keywords: Autoimmune disease; Cell therapy; Mesenchymal stem cells; Perinatal tissues; Psoriasis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Humans
Interleukin-17 / metabolism
Mesenchymal Stem Cells*
Mice
Psoriasis* / metabolism
Psoriasis* / therapy
Quality of Life
Umbilical Cord

Substances

Interleukin-17