Depletion and reconstitution of macrophages in mice with vesicular stomatitis virus infection

Yingchun Wang; Lifen Gao

doi:10.1016/j.xpro.2023.102319

Depletion and reconstitution of macrophages in mice with vesicular stomatitis virus infection

STAR Protoc. 2023 May 16;4(2):102319. doi: 10.1016/j.xpro.2023.102319. Online ahead of print.

Authors

Yingchun Wang¹, Lifen Gao²

Affiliations

¹ Key Laboratory for Experimental Teratology of Ministry of Education, Shandong Key Laboratory of Infection and Immunity, and Department of Immunology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China.
² Key Laboratory for Experimental Teratology of Ministry of Education, Shandong Key Laboratory of Infection and Immunity, and Department of Immunology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China. Electronic address: glfflg@sdu.edu.cn.

Abstract

Acting as the first line of host defense, macrophages play a central role in antiviral response. Here, we present a protocol for depletion and reconstitution of macrophages in mice with vesicular stomatitis virus (VSV) infection. We describe steps for induction and isolation of peritoneal macrophages from CD45.2⁺ donor mice, macrophage depletion in CD45.1⁺ recipient mice, adoptive transfer of CD45.2⁺ macrophages to CD45.1⁺ recipient mice, and VSV infection. This protocol highlights the role of exogenous macrophages in antiviral response in vivo. For complete details on the use and execution of this profile, please refer to Wang et al.¹.

Keywords: Cell isolation; Immunology.