Self-Assembly of Peptide-Lipid Nanoparticles for the Efficient Delivery of Nucleic Acids

Jiaojiao Zhang; Zixuan Wang; Jiwei Min; Xuelin Zhang; Rongxin Su; Yuefei Wang; Wei Qi

doi:10.1021/acs.langmuir.3c00834

Self-Assembly of Peptide-Lipid Nanoparticles for the Efficient Delivery of Nucleic Acids

Langmuir. 2023 May 30;39(21):7484-7494. doi: 10.1021/acs.langmuir.3c00834. Epub 2023 May 17.

Authors

Jiaojiao Zhang¹, Zixuan Wang¹, Jiwei Min¹, Xuelin Zhang¹, Rongxin Su^{1

2

3}, Yuefei Wang^{1

3}, Wei Qi^{1

2

3}

Affiliations

¹ State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P. R. China.
² Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, P. R. China.
³ Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin University, Tianjin 300072, P. R. China.

PMID: 37195813
DOI: 10.1021/acs.langmuir.3c00834

Abstract

A transfection formulation is successfully developed to deliver nucleic acids by adding an auxiliary lipid (DOTAP) to the peptide, and the transfection efficiency of pDNA reaches 72.6%, which is close to Lipofectamine 2000. In addition, the designed KHL peptide-DOTAP complex exhibits good biocompatibility by cytotoxicity and hemolysis analysis. The mRNA delivery experiment indicates that the complex had a 9- or 10-fold increase compared with KHL or DOTAP alone. Intracellular localization shows that KHL/DOTAP can achieve good endolysosomal escape. Our design provides a new platform for improving the transfection efficiency of peptide vectors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Liposomes
Nucleic Acids*
Peptides

Substances

Lipid Nanoparticles
Nucleic Acids
1,2-dioleoyloxy-3-(trimethylammonium)propane
Liposomes
Peptides