Green extraction and purification of chondroitin sulfate from jumbo squid cartilage by a novel procedure combined with enzyme, ultrasound and hollow fiber dialysis

Ming-Fong Tsai; Chun-Yung Huang; Parushi Nargotra; Wen-Rui Tang; Kuan-Ting Liao; Yi-Chen Lee; Chia-Min Lin; Chitsan Lin; Chwen-Jen Shieh; Chia-Hung Kuo

doi:10.1007/s13197-023-05701-7

Green extraction and purification of chondroitin sulfate from jumbo squid cartilage by a novel procedure combined with enzyme, ultrasound and hollow fiber dialysis

J Food Sci Technol. 2023 Jun;60(6):1711-1722. doi: 10.1007/s13197-023-05701-7. Epub 2023 Apr 10.

Authors

Affiliations

¹ Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung, 811 Taiwan.
² Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung, 811 Taiwan.
³ Biotechnology Center, National Chung Hsing University, Taichung, 402 Taiwan.
⁴ Center for Aquatic Products Inspection Service, National Kaohsiung University of Science and Technology, Kaohsiung, 811 Taiwan.

^# Contributed equally.

PMID: 37187986
PMCID: PMC10169932 (available on 2024-06-01)
DOI: 10.1007/s13197-023-05701-7

Abstract

Chondroitin sulfate (ChS) from marine sources is gaining attention. The purpose of this study was to extract ChS from jumbo squid cartilage (Dosidicus gigas) using ultrasound-assisted enzymatic extraction (UAEE). An ultrasound with protease assistance, including either alcalase, papain or Protin NY100 was used to extract ChS. The results showed that alcalase had the best extraction efficiency. The response surface methodology was employed to evaluate the relationship between extraction conditions and extraction yield of ChS. The ridge max analysis revealed a maximum extraction yield of 11.9 mg ml^{- 1} with an extraction temperature of 59.40 °C, an extraction time of 24.01 min, a pH of 8.25, and an alcalase concentration of 3.60%. Compared to ethanol precipitation, purification using a hollow fiber dialyzer (HFD) had a higher extraction yield of 62.72% and purity of 85.96%. The structure characteristics of ChS were identified using FTIR, ¹ H-NMR, and ¹³ C-NMR to confirm that the purified ChS structure was present in the form of chondroitin-4-sulfate and chondroitin-6-sulfate. The results of this study provide a green and efficient process for extraction and purification of ChS and are essential for the use of ChS for the development and production of nutrient food products or pharmaceuticals.

Supplementary information: The online version contains supplementary material available at 10.1007/s13197-023-05701-7.

Keywords: Chondroitin sulfate; Hollow fiber dialyzer; Jumbo squid cartilage; Protease; Response surface methodology; Ultrasound-assisted enzymatic extraction.

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