Authentication of olive oil in commercial products using specific, sensitive, and rapid loop-mediated isothermal amplification

Shyang-Chwen Sheu; Ying-Jie Wang; Pao-Cheng Huang; Yi-Yang Lien; Meng-Shiou Lee

doi:10.1007/s13197-023-05726-y

Authentication of olive oil in commercial products using specific, sensitive, and rapid loop-mediated isothermal amplification

J Food Sci Technol. 2023 Jun;60(6):1834-1840. doi: 10.1007/s13197-023-05726-y. Epub 2023 Mar 21.

Authors

Shyang-Chwen Sheu¹, Ying-Jie Wang¹, Pao-Cheng Huang¹, Yi-Yang Lien², Meng-Shiou Lee³

Affiliations

¹ Department of Food Science, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan.
² Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201 Taiwan.
³ Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung, 40402 Taiwan.

PMID: 37187977
PMCID: PMC10169996 (available on 2024-06-01)
DOI: 10.1007/s13197-023-05726-y

Abstract

Olive oil is an important and popularly used plant oil in the daily diet or chemical industry. Due to its biological benefits on human health and higher selling prices, adulteration of olive oil for commercial fraud by other plant oils is becoming a serious issue. In this study, a specific, sensitive and rapid loop-mediated isothermal amplification (LAMP) was first developed for the detection of Olea europaea DNA for olive oil authentication. The oleosin gene was used for the primer design of the LAMP assay. After primer validation, the results showed that the LAMP primers were specific and rapid to isothermally authenticate the oleosin gene of Olea europaea within 1 h at 62 °C and had no cross-reaction with other DNA of plant oils. The sensitivity of LAMP was 1 ng of genomic DNA in olive oil, and only 1% olive oil in the sample was requisite during DNA amplification. Additionally, positive detection by LAMP in all the collected commercial olive oil products was practically performed but not in PCR assays. In conclusion, herein, the established LAMP assay with specificity could not only be capable for rapid identification but also applicable for olive oil authentication for precluding adulteration in plant oil products.

Supplementary information: The online version contains supplementary material available at 10.1007/s13197-023-05726-y.

Keywords: DNA authentication; Loop-mediated isothermal amplification (LAMP); Olea europaea; Olive oil.

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