Telomere-to-telomere genome assembly of matsutake (Tricholoma matsutake)

Hiroyuki Kurokochi; Naoyuki Tajima; Mitsuhiko P Sato; Kazutoshi Yoshitake; Shuichi Asakawa; Sachiko Isobe; Kenta Shirasawa

doi:10.1093/dnares/dsad006

Telomere-to-telomere genome assembly of matsutake (Tricholoma matsutake)

DNA Res. 2023 Apr 25;30(3):dsad006. doi: 10.1093/dnares/dsad006. Online ahead of print.

Authors

Hiroyuki Kurokochi¹, Naoyuki Tajima², Mitsuhiko P Sato², Kazutoshi Yoshitake³, Shuichi Asakawa³, Sachiko Isobe², Kenta Shirasawa²

Affiliations

¹ Department of Forest Science, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
² Department of Frontier Research and Development, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
³ Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.

Abstract

Here, we report the first telomere-to-telomere genome assembly of matsutake (Tricholoma matsutake), which consists of 13 sequences (spanning 161.0 Mb) and a 76 kb circular mitochondrial genome. All the 13 sequences were supported with telomeric repeats at the ends. GC-rich regions are located at the middle of the sequences and are enriched with long interspersed nuclear elements (LINEs). Repetitive sequences including long-terminal repeats (LTRs) and LINEs occupy 71.6% of the genome. A total of 21,887 potential protein-coding genes were predicted. The genomic data reported in this study served not only matsutake gene sequences but also genome structures and intergenic sequences. The information gained would be a great reference for exploring the genetics, genomics, and evolutionary study of matsutake in the future, and ultimately facilitate the conservation of this vulnerable genetic resource.

Keywords: Genome assembly; Long-read sequencing technology; Telomere-to-telomere.