Irrational use of fluoroquinolones (FQs) can lead to allergic reactions, adverse reactions to the heart and damage of the liver; thus, it is of great significance to establish rapid, sensitive and accurate detection methods for FQs. Molecularly imprinted polymers (MIPs) with specific structures synthesized by molecular imprinting technology (MIT) are widely used for the detection of FQs due to their high specificity, high sensitivity and stable performance. Recently, new functional nanomaterials with different morphologies and sizes, which can provide rich sites for surface chemical reactions, have attracted more and more attention of the researchers. Thus, the application status and development prospects of MIT based on new nanomaterials in the adsorption and detection of FQs were summarized in this study, providing a theoretical basis and technical guarantee for the development of new and efficient food safety analysis strategies based on MIPs.