OMIP-91: A 27-color flow cytometry panel to evaluate the phenotype and function of human conventional and unconventional T-cells

Jeremy Anderson; Sedi Jalali; Paul V Licciardi; Daniel G Pellicci

doi:10.1002/cyto.a.24738

OMIP-91: A 27-color flow cytometry panel to evaluate the phenotype and function of human conventional and unconventional T-cells

Cytometry A. 2023 Jul;103(7):543-547. doi: 10.1002/cyto.a.24738. Epub 2023 May 14.

Authors

Jeremy Anderson^{1

2}, Sedi Jalali^{1

2

3}, Paul V Licciardi^{1

2}, Daniel G Pellicci^{1

3

4}

Affiliations

¹ New Vaccines, Murdoch Children's Research Institute, Melbourne, Australia.
² Department of Paediatrics, University of Melbourne, Melbourne, Australia.
³ Cellular Immunology, Murdoch Children's Research Institute, Melbourne, Australia.
⁴ Department of Microbiology and Immunology, University of Melbourne, Melbourne, Australia.

PMID: 37183268
DOI: 10.1002/cyto.a.24738

Abstract

This 27-color panel was developed to simultaneously measure different T-cell populations (CD4, CD8, γδ T-cells, and MAIT cells) and their subsets (Memory, Th1, Th2, Th17, Tfh, and Treg) along with functional markers associated with their activation status, cytokine production and cytotoxicity. This panel will be useful for both in vivo and in vitro studies evaluating T-cells in the context of human health and disease. This panel is valuable in settings where samples are limited as a large amount of data will be generated using small volumes of blood.

Keywords: PBMCs; T-cells; activation; intracellular cytokines.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cytokines
Flow Cytometry
Humans
Mucosal-Associated Invariant T Cells*
Phenotype
T-Lymphocyte Subsets
Th17 Cells*

Substances

Cytokines