Injectable chitosan/xyloglucan composite hydrogel with mechanical adaptivity and endogenous bioactivity for skin repair

Hua Wei; Shengkai Yu; Yansheng Zhang; Hua Zhang; Yuxi Ma; Mengjia Xu; Peng An; Yang Zhou; Sami Halila; Yen Wei; Jing Chen

doi:10.1016/j.carbpol.2023.120904

Injectable chitosan/xyloglucan composite hydrogel with mechanical adaptivity and endogenous bioactivity for skin repair

Carbohydr Polym. 2023 Aug 1:313:120904. doi: 10.1016/j.carbpol.2023.120904. Epub 2023 Apr 13.

Authors

Hua Wei¹, Shengkai Yu², Yansheng Zhang³, Hua Zhang², Yuxi Ma³, Mengjia Xu⁴, Peng An⁴, Yang Zhou⁴, Sami Halila⁵, Yen Wei⁶, Jing Chen⁷

Affiliations

¹ Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
² Research Institute for Medical and Biological Engineering, Ningbo University, Ningbo 315211, China.
³ Zhejiang Engineering Research Center for Biomedical Materials, Zhejiang International Scientific and Technological Cooperative Base of Biomedical Materials and Technology, Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315300, China; University of Chinese Academy of Sciences, Beijing 100049, China.
⁴ Zhejiang Engineering Research Center for Biomedical Materials, Zhejiang International Scientific and Technological Cooperative Base of Biomedical Materials and Technology, Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315300, China.
⁵ Université Grenoble Alpes, CNRS, CERMAV, Grenoble 38000, France. Electronic address: sami.halila@cermav.cnrs.fr.
⁶ Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing 100084, China. Electronic address: weiyen@mail.tsinghua.edu.cn.
⁷ Institute of Medical Sciences, The Second Hospital and Shandong University Center for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan 250033, China. Electronic address: jc@email.sdu.edu.cn.

PMID: 37182937
DOI: 10.1016/j.carbpol.2023.120904

Abstract

Delayed or chronic wound healing is one of severe clinical issues. Developing scaffold materials capable of supporting cells and inducing tissue regeneration remains a challenge. Here, a polysaccharide-based hydrogel is constructed for promoting full-thickness skin wound healing in mouse model. The engineering hydrogel consists of a dynamic crosslinking network formed by the Schiff base reaction between aldehyde-containing xyloglucan and methacrylated chitosan. Its reversible gel-sol-gel transition upon shearing force is highly beneficial to completely cover and fill irregular wound shape. The second covalent cross-linking network achieved by photo-initiated polymerization offers a feasible way to tune the mechanical property of hydrogel after injection, with an ideal mechanical adaptivity for clinical application. Remarkably, both in vitro and in vivo evaluations demonstrate that the hydrogel with endogenously bioactive galactoside units can promote cell spheroid formation and accelerate wound healing by expediting re-epithelialization, collagen deposition, angiogenesis as well as the formation of hair follicles.

Keywords: Bioactive polysaccharides; Dynamically covalent bonds; Injectable hydrogels; Wound healing.

MeSH terms

Animals
Chitosan* / pharmacology
Glucans / pharmacology
Hydrogels / pharmacology
Mice
Wound Healing

Substances

Chitosan
Hydrogels
xyloglucan
Glucans