A conductometric enzymatic methanol sensor based on polystyrene - PAMAM dendritic polymer electrospun nanofibers

Pouyan Razmshoar; Fatma Besbes; Anis Madaci; Rym Mlika; S Hajir Bahrami; Mohammad Rabiee; Marie Martin; Abdelhamid Errachid; Nicole Jaffrezic-Renault

doi:10.1016/j.talanta.2023.124630

A conductometric enzymatic methanol sensor based on polystyrene - PAMAM dendritic polymer electrospun nanofibers

Talanta. 2023 Aug 1:260:124630. doi: 10.1016/j.talanta.2023.124630. Epub 2023 May 3.

Authors

Pouyan Razmshoar¹, Fatma Besbes², Anis Madaci³, Rym Mlika⁴, S Hajir Bahrami⁵, Mohammad Rabiee⁶, Marie Martin³, Abdelhamid Errachid³, Nicole Jaffrezic-Renault⁷

Affiliations

¹ Textile Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran; University of Lyon, Institute of Analytical Sciences, UMR 5280, CNRS, F-69100, Villeurbanne, France.
² University of Lyon, Institute of Analytical Sciences, UMR 5280, CNRS, F-69100, Villeurbanne, France; University of Monastir, Laboratory of Interfaces and Advanced Materials, Faculty of Science of Monastir, 5019, Monastir, Tunisia.
³ University of Lyon, Institute of Analytical Sciences, UMR 5280, CNRS, F-69100, Villeurbanne, France.
⁴ University of Monastir, Laboratory of Interfaces and Advanced Materials, Faculty of Science of Monastir, 5019, Monastir, Tunisia.
⁵ Textile Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
⁶ Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
⁷ University of Lyon, Institute of Analytical Sciences, UMR 5280, CNRS, F-69100, Villeurbanne, France. Electronic address: nicole.jaffrezic@univ-lyon1.fr.

PMID: 37178675
DOI: 10.1016/j.talanta.2023.124630

Abstract

Methanol (MeOH) is a solvent and cleaning agent used in industry, but it is poisonous when ingested. The recommended release threshold for MeOH vapor is 200 ppm. We present a novel sensitive micro-conductometric MeOH biosensor created by grafting alcohol oxidase (AOX) onto electrospun polystyrene-poly(amidoamine) dendritic polymer blend nanofibers (PS-PAMAM-ESNFs) on interdigitated electrodes (IDEs). The analytical performance of the MeOH microsensor was evaluated using gaseous MeOH, ethanol, and acetone samples collected from the headspace above aqueous solution with known concentration. The sensor's response time (t_Res) fluctuates from 13 s to 35 s from lower to higher concentrations. The conductometric sensor has a sensitivity of 150.53 μS.cm-1 (v/v) for MeOH and a detection limit of 100 ppm in the gas phase. The MeOH sensor is 7.3 times less sensitive to ethanol and 136.8 times less sensitive to acetone. The sensor was verified for detecting MeOH in commercial rubbing alcohol samples.

Keywords: Alcohol oxidase; Dendrimer; Methanol sensor; Micro-conductometric sensor; Nanofibers; Rubbing alcohol.