A triphenylamine-based fluorescence probe for detection of hypochlorite in mitochondria

Guan-Hua Yu; Hao-Ran Hu; Rui-Bin Liu; Guo-Zhu Sheng; Jia-Jie Niu; Ying Fang; Kun-Peng Wang; Zhi-Qiang Hu

doi:10.1016/j.saa.2023.122830

A triphenylamine-based fluorescence probe for detection of hypochlorite in mitochondria

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Oct 15:299:122830. doi: 10.1016/j.saa.2023.122830. Epub 2023 May 10.

Authors

Guan-Hua Yu¹, Hao-Ran Hu¹, Rui-Bin Liu², Guo-Zhu Sheng², Jia-Jie Niu², Ying Fang³, Kun-Peng Wang⁴, Zhi-Qiang Hu⁵

Affiliations

¹ Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
² Shandong Kangqiao Biotechnology Co. Ltd, Binzhou 256500, China.
³ Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: fangyingqust@126.com.
⁴ Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: wangkunpeng@qust.edu.cn.
⁵ Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: huzhiqiang@qust.edu.cn.

PMID: 37178586
DOI: 10.1016/j.saa.2023.122830

Abstract

The level of HClO/ClO^- in mitochondria is essential to keep the normal function of mitochondria. Therefore, it is meaningful to accurately and quickly monitor ClO^- in mitochondria. In this work, a new triphenylamine-based fluorescence probe PDTPA was designed and synthesized, in which pyridinium salt and dicyano-vinyl group were introduced as mitochondria targeting site and reaction site for ClO^-. The probe showed high sensitivity and fast fluorescence response (<10 s) in the detection of ClO^-. Moreover, the probe PDTPA had good linearity in a wide concentration range of ClO^- and its detection limit was calculated as 10.5 μM. Confocal fluorescence images demonstrated that the probe could target mitochondria and track the fluctuations of endogenous/exogenous ClO^- levels in the mitochondria of living cells.

Keywords: ClO(−) detection; Fluorescence probe; Mitochondrial targeting; Triphenylamine.

MeSH terms

Fluorescent Dyes*
HeLa Cells
Humans
Hypochlorous Acid*
Microscopy, Fluorescence / methods
Mitochondria

Substances

Hypochlorous Acid
Fluorescent Dyes