Hybrid Nanomaterial of Graphene Oxide Quantum Dots with Multi-Walled Carbon Nanotubes for Simultaneous Voltammetric Determination of Four DNA Bases

Qusai Hassan; Chevon Riley; Meissam Noroozifar; Kagan Kerman

doi:10.3390/nano13091509

Hybrid Nanomaterial of Graphene Oxide Quantum Dots with Multi-Walled Carbon Nanotubes for Simultaneous Voltammetric Determination of Four DNA Bases

Nanomaterials (Basel). 2023 Apr 29;13(9):1509. doi: 10.3390/nano13091509.

Authors

Qusai Hassan^{1

2}, Chevon Riley¹, Meissam Noroozifar¹, Kagan Kerman^{1

2}

Affiliations

¹ Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada.
² Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada.

Abstract

In this proof-of-concept study, a novel hybrid nanomaterial-based electrochemical sensor was developed for the simultaneous detection of four DNA bases. For the modification of the working electrode surface, graphene oxide quantum dots (GOQDs) were synthesized using a solvothermal method. GOQDs were then used for the preparation of a hybrid nanomaterial with multi-walled carbon nanotubes (GOQD-MWCNT) using a solvothermal technique for the first time. Transmission electron microscopy (TEM) was used to characterize the GOQDs-MWCNTs. A glassy carbon electrode (GCE) was modified with the GOQDs-MWCNTs using Nafion™ to prepare a GOQD-MWCNT/GCE for the simultaneous determination of four DNA bases in phosphate buffer solution (PBS, pH 7.0) using differential pulse voltammetry (DPV). The calibration plots were linear up to 50, 50, 500, and 500 µM with a limit of detection at 0.44, 0.2, 1.6, and 5.6 µM for guanine (G), adenine (A), thymine (T) and cytosine (C), respectively. The hybrid-modified sensor was used for the determination of G, A, T, and C spiked in the artificial saliva samples with the recovery values ranging from 95.9 to 106.8%. This novel hybrid-modified electrochemical sensor provides a promising platform for the future development of a device for cost-effective and efficient simultaneous detection of DNA bases in real biological and environmental samples.

Keywords: DNA; electrochemical sensor; graphene oxide quantum dots; hybrid nanomaterial; multi-walled carbon nanotubes; simultaneous determination.

Grants and funding

This work was supported by the Canada Research Chair Tier-2 award to K. Kerman for “Bioelectrochemistry of Proteins” (Project No. 950-231116), the Ontario Ministry of Research and Innovation (Project No. 35272), Discovery Grant (Project No. RGPIN-2020-07164) from the Natural Sciences and Engineering Research Council of Canada (NSERC), and the Canada Foundation for Innovation (Project No. 35272).