Optimization of Extraction and Separation Process of Notoginsenoside Fc from Panax notoginseng Leaves

Zhenghong Guo; Zhonghua Luo; Shao Wu; Chunhong Yang; Ting Xiao; Yuqing Zhao

doi:10.3390/molecules28093915

Optimization of Extraction and Separation Process of Notoginsenoside Fc from Panax notoginseng Leaves

Molecules. 2023 May 5;28(9):3915. doi: 10.3390/molecules28093915.

Authors

Zhenghong Guo¹, Zhonghua Luo², Shao Wu², Chunhong Yang², Ting Xiao³, Yuqing Zhao⁴

Affiliations

¹ College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China.
² China School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, China.
³ The State Key Laboratory of Functions and Applications of Medicinal Plants, The Department of Pharmaceutic Preparation of Chinese Medicine, The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Druggability, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550025, China.
⁴ Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Yanbian University, Yanji 133002, China.

Abstract

Response surface methodology (RSM) was used to determine the optimal conditions for ultrasound-assisted extraction (UAE) of Notoginsenoside Fc (Fc) from panax notoginseng leaves. The experiment utilized a Box-Behnken design (BBD) and separation conditions were optimized. The optimum extraction conditions were as follows: extraction time = 1.5 h, ethanol concentration = 86%, liquid-to-solid ratio = 19:1. The experimentally obtained values were in accordance with the values predicted by the RSM model. We determined that the RSM model was able to successfully simulate the optimal extraction of Fc from the leaves. Further, Fc was enriched from Panax notoginseng through nine macroporous resins, and HPD-100 macroporous resins were selected for preliminary enrichment of Fc due to its economic costs and benefits. Subsequently, octadecyl silane (ODS) column chromatography was used to improve the purity of Fc to over 90% after separation by ODS column chromatography. Fc with a purity greater than 95% can be obtained by recrystallization. This is the first study that has focused on the extraction and enrichment of Fc from Panax notoginseng leaves using macroporous resin combined with ODS column chromatography, which provides the possibility for further application of Fc.

Keywords: Notoginsenoside Fc; Panax notoginseng leaves; enrichment; extraction optimization.

MeSH terms

Chromatography, High Pressure Liquid
Drugs, Chinese Herbal* / chemistry
Ginsenosides* / analysis
Panax notoginseng* / chemistry
Panax*
Plant Leaves / chemistry

Substances

notoginsenoside Fc
Ginsenosides
Drugs, Chinese Herbal

Grants and funding

This research was supported by the Technology Platform of Industrialization Chromatographic Preparation for Standard Extract of Traditional Chinese Medicine (2010ZX09401-304-105B) and Liaoning (FGW) Engineering Technology Research Center for Industrial Chromatographic Preparation of Natural Innovative Drug Materials (2017–1007).