Spatio-temporal pattern of c-Jun N-terminal kinase isoforms in the cochleae of C57BL/6J mice with presbycusis

Rui Ding; Yi Pan; Kaili Chen; Tianyuan Zou; Andi Zhang; Dongye Guo; Peilin Ji; Cui Fan; Bin Ye; Mingliang Xiang

doi:10.1016/j.heares.2023.108784

Spatio-temporal pattern of c-Jun N-terminal kinase isoforms in the cochleae of C57BL/6J mice with presbycusis

Hear Res. 2023 Jul:434:108784. doi: 10.1016/j.heares.2023.108784. Epub 2023 May 6.

Authors

Rui Ding¹, Yi Pan¹, Kaili Chen¹, Tianyuan Zou¹, Andi Zhang¹, Dongye Guo¹, Peilin Ji¹, Cui Fan¹, Bin Ye², Mingliang Xiang³

Affiliations

¹ Department of Otolaryngology & Head and Neck Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Ear Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
² Department of Otolaryngology & Head and Neck Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Ear Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: yb12237@rjh.com.cn.
³ Department of Otolaryngology & Head and Neck Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Ear Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: mingliangxiang@163.com.

PMID: 37172415
DOI: 10.1016/j.heares.2023.108784

Abstract

The c-Jun N-terminal kinase (JNK) pathway is a vital component of the mitogen-activated protein kinase cascade, which regulates cell death and survival. The present study aimed to explore the Spatio-temporal changes in all JNK isoforms in the cochleae of C57/BL6J mice with age-related hearing loss. Changes in the three isoforms of JNKs in the cochleae of an animal model with presbycusis and the senescent HEI-OC1 cell line were tested by immunohistochemistry staining and western blotting. Our results demonstrated that all three JNK isoforms are distributed in the cochleae, and the expression patterns of JNK1, JNK2, and JNK3 differed in hair cells, spiral ganglion neurons, and stria vascularis, with great significance in the cochleae of adult C57BL/6J mice. The levels of JNK1, JNK2, and JNK3 showed various spatio-temporal changes in the aging mice. In a senescent hair cell model, changes in JNK1, JNK2, and JNK3 expression levels were similar to those observed in the cochleae. Our study is the first to show that JNK3 is highly expressed in the hair cells of C57BL/6J mice and further increases in conjunction with age-related hearing loss, suggesting that it may play a more critical role than previously believed in hair cell loss and spiral ganglion degeneration.

Keywords: Hair cell; JNK; Presbycusis; Spiral ganglion; Stria vascularis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
JNK Mitogen-Activated Protein Kinases* / metabolism
Mice
Mice, Inbred C57BL
Mitogen-Activated Protein Kinase 8 / genetics
Mitogen-Activated Protein Kinase 8 / metabolism
Presbycusis* / genetics
Protein Isoforms

Substances

JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinase 8
Protein Isoforms