Timing of isolation from an enriched environment determines the level of aggressive behavior and sexual maturity in Siamese fighting fish (Betta splendens)

Eri Iwata; Kyouhei Masamoto; Hiroyuki Kuga; Miho Ogino

doi:10.1186/s40850-021-00081-x

Timing of isolation from an enriched environment determines the level of aggressive behavior and sexual maturity in Siamese fighting fish (Betta splendens)

BMC Zool. 2021 May 10;6(1):15. doi: 10.1186/s40850-021-00081-x.

Authors

Eri Iwata^{1

2

3}, Kyouhei Masamoto⁴, Hiroyuki Kuga⁴, Miho Ogino⁵

Affiliations

¹ Department of Science and Technology, Iwaki Meisei University, 5-5-1 Chuoudai, Ihino, Iwaki, Fukushima, 970-8551, Japan. e-iwata@vet.ous.ac.jp.
² Institute of Osaka Marine Research, 19-34, Shimodachou, Nishi Ku, Sakai City, Osaka, 593-8329, Japan. e-iwata@vet.ous.ac.jp.
³ Faculty of Veterinary Medicine, Okayama University of Science, 1-3, Ikoinooka, Imabari City, Ehime, 794-8555, Japan. e-iwata@vet.ous.ac.jp.
⁴ Department of Science and Technology, Iwaki Meisei University, 5-5-1 Chuoudai, Ihino, Iwaki, Fukushima, 970-8551, Japan.
⁵ Faculty of Veterinary Medicine, Okayama University of Science, 1-3, Ikoinooka, Imabari City, Ehime, 794-8555, Japan.

Abstract

Background: Teleost fish are known to respond to environmental manipulation, which makes them an ideal model animal for testing relationships between the environment and behavior. The Siamese fighting fish, Betta splendens, is a solitary, highly territorial fish that displays fierce stereotyped aggressive behavior toward conspecifics or members of other species. Adult fish, especially males, are generally housed in isolation in captivity. Here we report evidence that an enriched rearing environment can decrease the level of aggression in bettas and enable adults to be housed in groups.

Results: B. splendens individuals were hatched in our laboratory and raised in groups in an enriched environment. At the juvenile or subadult stage, some individuals were relocated to a poor environment and kept in isolation. To evaluate aggression, a mirror-image test was conducted at the juvenile, subadult, and adult stages for each fish, and body parameters as well as plasma concentrations of 11-ketotestosterone, estradiol, and cortisol were evaluated. Male and female adult bettas raised in a group showed lower levels of aggression than other adult fish. The magnitude of threatening behavior was greater in adult bettas isolated as subadults, whereas the magnitude of fighting behavior was grater in adult bettas isolated as juveniles. The influence of rearing conditions on behavior was greater in females than in males. Plasma cortisol concentrations of adult bettas isolated as subadults after the mirror-image test were higher than those in other experimental groups. Adult males isolated as subadults had significantly higher plasma concentrations of 11-ketotestosterone than males raised in a group and isolated as juveniles. Females isolated as subadults had a higher gonadosomatic index than females raised in a group and females isolated as juveniles.

Conclusions: These results indicate that bettas can be kept in a group under enriched environments and that the timing of isolation influences the aggression and sexual maturity of bettas. Female and male bettas responded differently to environmental manipulation. Judging from their level of sexual maturity, bettas isolated as subadults show proper development.

Keywords: Aggression; Betta splendens; Environmental enrichment; Fighting fish; Maturity.